IN VITRO CALLUS INDUCTION AND COMPARATIVE GC-MS ANALYSIS OF METHANOLIC EXTRACTS OF CALLUS AND LEAF SAMPLES OF AMPELOCISSUS LATIFOLIA (ROXB.) PLANCH
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 9
Abstract
Objective: The aim of the present study was to develop a callus induction protocol and comparative study of therapeutic phytochemicals present in in vivo leaf and in vitro callus extracts through Gas Chromatography-Mass Spectrometry analysis.Methods: Murashige and Skoog media was used as culture media for callus induction. In vitro callus induction protocol was developed by studying the effects of various plant growth regulators like auxin, 2, 4-D (2,4-dichlorophenoxyacetic acid), NAA (naphthalic acetic acid), alone and in combination with cytokinin BAP (benzyl aminopurine), on leaf and stem explants. The GC-MS analysis of Ampelocissus latifolia was carried out on Shimadzu QP-2010 plus with thermal desorption system TD 20 to study the phytochemical profile.Results: In vitro callus induction protocol was developed for the plant and callusing was done from leaf and stem explants of Ampelocissus latifolia. The best result for callus induction was obtained using leaf explant, and callus production were maximum in Murashige and Skoog medium fortified with BAP (0.5 mg/l) and NAA (1.0 mg/l). Major compounds identified in the GC-MS analysis were Campesterol, Stigmasterol, Beta-Sitosterol, Docosanol, Dodecanoic acid, etc., in in vitro extract and Beta Sitosterol, Tocopherol, Squalene, Bergamot oil, Margarinic acid, Hexadecanoic acid, etc., in in vivo extract. The different active phytochemicals identified have been found to possess a wide range of biological activities, thus this analysis forms a basis for the biological characterization and importance of the compounds identified for human benefits.Conclusion: This is the first report on callus induction in Ampelocissus latifolia. From the results obtained through the in vitro callus induction and its comparative GCMS analysis with in vivo extract, it is revealed that Ampelocissus latifolia contains various bioactive compounds that are of importance for phytopharmaceutical uses. The GCMS analysis revealed that the amount of Beta-sitosterol and 5-Hydroxymethylfurfural (HMF) was very high in in vitro extract as compared to in vivo extract.
Authors and Affiliations
Deep Chhavi Anand, Rishikesh Meena, Vidya Patni
Keywords
Related Articles
- EP ID EP566160
- DOI 10.22159/ijpps.2018v10i9.27879
- Views 75
- Downloads 0
QUALITY CONTROL STANDARDIZATION OF THE BARK OF MORINGA OLEIFERA LAM.
Objective: Moringa oleifera Lam. (Morangaceae) is a large glabrous tree, found throughout India. It is well known for curing a variety of ailments such as wound Healing, dysentery, fever, diarrhea and urinary problems. T...
METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS DETERMINATION OF STEVIOSIDE, REBAUDIOSIDE-A, REBAUDIOSIDE C AND DULCOSIDE A CONTAINED IN STEVIA REBAUDIANA BERTONI USING HPLC-ELSD
Objective: To develop and validate a selective HPLC-ELSD method for determination of steviol glycosides contained in Stevia rebaudiana, mainly stevioside, rebauside A, rebaudioside C, and dulcoside A. Methods: The chroma...
NEVIRAPINE INDUCED STEVENS JOHNSON SYNDROME: A CASE REPORT
Adverse drug reactions (ADRs) are one of the major reasons for morbidity and mortality in India, but they often go undetected and under reported. Nevirapine (NVP) is one of the first line agents used for anti retroviral...
EXISTING EVIDENCE FOR SAFE USE OF HERBAL MEDICINES IN CHRONIC KINEY DISEASE
There has been a widespread use of herbal medicines in the last two decades, despite a lack of scientific evidence for their efficacy and safety. Health care professionals have expressed growing concerns about safety iss...
PROPERTIES OF GUAR GUM - DEAD SEA SALT (GG-DSS) MEDICATED GEL
Objective: The main objective of this study is to understand the viscoelastic nature of Guar Gum - Dead Sea Salt (GG-DSS) based polymeric gel, a semisolid product; which is prepared with all natural ingredients to achiev...