In vitro conservation of rare, medicinally important species Dendrobum nobile Lindl. (Orchidaceae)
Journal Title: Annals of Plant Sciences - Year 2018, Vol 7, Issue 2
Abstract
A comparative study was made to access the regeneration response of the stem-node discs of Dendrobium nobile procured from in vivo (greenhouse) grown plants and in vitro grown cultures. The response of stem-node discs, procured both from in vivo and in vitro source, was successfully tested in M (Mitra et al., 1976) medium alone and in combinations with cytokinins [N6-benzyladenine (6-BA - 0.5, 1.0 mg l-1), furfurylaminopurine (Kn - 0.5, 1.0 mg l-1) and auxin [α-naphthalene acetic acid (NAA - 0.5, 1.0 mg l-1)]. The explants regenerated via shoot bud initiation in the cultures. Juvenility of the tissues and chemical stimulus were the major factors in initiating the response in the explants. The initiation of regeneration response in in vitro derived explants was not obligatory to a treatment with growth regulators as compared to those derived from in vivo ones. Cytokinins at 1.0 mg l-1 favoured optimum regeneration percentage in the explants and invoked additional supernumerary loci in the regenerants. The regenerants from in vitro derived explants developed plantlets early in cytokinins enriched combinations.
Authors and Affiliations
Saranjeet Kaur
Keywords
Related Articles
- EP ID EP280664
- DOI 10.21746/aps.2018.7.3.6
- Views 113
- Downloads 0
Distribution and population dynamics of Calamus nambariensis Becc. - An endemic and threatened cane of Assam
Rattans are prickly climbing palm which comprise more than fifty percent of the total palm taxa found in India. This non-timber forest product has great economic importance in handicraft and furniture making because of i...
Micro-morphological descriptions on Cenchrus species from Rajasthan (India)
Present investigation was carried out to screen micro-morphological features of Cenchrus species (C4 plant) belonging to family Poaceae. This is an important medicinal, fodder as well as crop plant and traditionally used...
Perpetuation of rice blast pathogen (Magnaporthe grisea) under temperate conditions of Kashmir, India.
Investigations were carried out during the months of October to March on various common specimens viz, paddy straw, stubbles, weed straw, soil, rice residue (husk), seeds and undecomposed organic material to ascertain th...
Morphotaxonomical studies on some mosses of Indian Thar desert
Studies on the mosses of hot and arid Indian Thar Desert revealed the occurrence of five moss species viz. Tortula muralis Hedw., Gymnostomiella veronicosa (Hook.) Fleisch., Hyophila involuta (Hook.) Jaeger., Semibarbula...
On the occurrence of two little known Fimbristylis (Cyperaceae) from Kerala, India.
Two little known Fimbristylis species have been collected from lateritic plains of Kerala. A brief description, notes and detailed illustrations are provided for easy identification.