In Vitro Cytoprotection of Resveratrol against H2O2-Induced Oxidative Stress and Injury in Astrocytes
Journal Title: Journal of Behavior Therapy and Mental Health - Year 2015, Vol 1, Issue 1
Abstract
Oxidative stress mediated neural cell death is thought to be involved in the progression of secondary cell injury following brain trauma. Agents that can block oxidative stress-related injury could be potential therapies for TBI. Resveratrol, a polyphenol found in plants and red wine, is cytoprotective due to its potent antioxidant activities. To further understand how resveratrol could affect oxidative stress-induced injury, we hypothesized that the cytoprotective activities of resveratrol could be dose-dependent. In this study, resveratrol-induced cytoprotection was evaluated in cultured astrocytes. Primary rat astrocytes were cultured in T-75 flasks to a confluence of 80% before being plated onto 96-well plates. After 24 hours of acclimation, astrocytes were treated with various doses of hydrogen peroxide (H2O2) (0.1, 0.25, 0.5 and 1 µM) and resveratrol (25, 50, 75, 100 µM), respectively. Cell viability was determined 24 hours later using Alamar Blue Assay. Treatment of astrocytes with 0.5 mM H2O2, left 65% of astrocytes non-viable whereas treatment of astrocytes with 0.1 mM H2O2 had no effect on astrocytes viability; whereas 1 mM, H2O2 caused total loss of astrocyte viability. Resveratrol treatment at 75 µM and 100 µM has reduced 0.5 mM H2O2-induced cytotoxicity in astrocytes by 50%. Immunostaining with GFAP also confirmed these findings about the cytoprotective effects of resveratrol in astrocytes exposed to H2O2. These results suggest that resveratrol could be a potential neuroprotective agent in TBI due to its antioxidant properties. Further studies are needed to evaluate the long- term effects of resveratrol in animal models of TBI.
Authors and Affiliations
Guoqiang Xing, Osarieme Evbuomwan, Zhaozhang Li, Pushpa Sharma
Keywords
Related Articles
- EP ID EP261959
- DOI 10.14302/issn.2474-9273.jbtm-16-1151
- Views 165
- Downloads 0
Influences of Australian Nursing Students’ Anxiety, Depression, Personality and Family iInteraction on their Psychological Well-Being and Suicidal Ideation
Objective: To examine the relationships between Australian nursing students’ anxiety, depression, personality and family interaction with psychological well-being and suicidal ideation. Participants: A sample of 201 n...
Multidisciplinary Doctor’s Clinical Practice, Confident and Difficulties to Diagnose, Manage and Care for Dementia in Remote Area Hospital
Objectives: To examine multidisciplinary doctor’s clinical practice, confident and difficulties to manage and care for dementia in remote area hospital. Method: A structured self-completed questionnaire, purposive sam...
Compassion Fatigue and Adopted Coping Strategies of Mental Health Service Providers Working in A Regional Psychiatric Hospital in Nigeria
Background: Mental health service providers sometimes suffer burden resulting from their care of mentally ill individuals, and this burden could be modulated by the coping mechanisms they adopt. Objective: This study...
Unsettled; Mental Stress in Community-Living Adolescents Who are Seeking Asylum in Australia
Objectives: This pilot study explored post-traumatic stress symptoms (PTSS) and moderate to severe psychological distress in a small sample of urban community-living adolescents seeking asylum in Australia. The study al...
Childhood Somatic Complaints: Relationships with Child Emotional Functioning and Parental Factors
Many schoolchildren experience somatic complaints such as headaches, abdominal pain and fatigue. The aim of the current research is to test the full model of previously found associations between negative affect and soma...