In vitro Cytotoxic Activity of Ethyl Acetate Fraction of Hibiscus vitifolius Flowers Against HeLa Cell Line
Journal Title: Tropical Journal of Natural Product Research - Year 2018, Vol 2, Issue 3
Abstract
Received 18 December 2017 Revised 04 February 2018 Accepted 24 February 2018 Published online 07 March 2018 Hibiscus vitifolius Linn.(syn. Fioria vitifolia (L.) Mattei), is a plant native of India and many other tropical regions. It has been used traditionally for the treatment of a number of diseases. It is also well known for its antioxidant activity. The objective of the present study was to perform a preliminary phytochemical analysis and cytotoxic activity of ethyl acetate extract of Hibiscus vitifolius (Malavaceae) flowers. The finely powdered flowers were extracted successively with petroleum ether, diethyl ether and ethyl acetate in a soxhlet apparatus for 72 h. The ethyl acetate fraction obtained was tested for the phytochemical constituents by using preliminary phytochemical tests. The cytotoxic activity of the extract was screened by MTT (methylthiazolyl diphenyl- tetrazolium bromide) assay against HeLa (Human cervical cancer) cell lines. The phytochemical analysis revealed the presence of triterpenoids, steroids, flavonoids, tannins and phenolic compounds. The extract was shown to inhibit the cell growth with IC50 value of 81.27 µg/mL. These findings suggest that the ethyl acetate fraction obtained from the flower extracts of Hibiscus vitifolius has moderate cytotoxic activity.
Authors and Affiliations
Jyothirmayi, Naga, Rahamat Unissa
Keywords
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- DOI 10.26538/tjnpr/v2i3.4
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