In-vitro cytotoxicity and Phytochemical Activity in Bryophyllum pinnatum
Journal Title: INTERNATIONAL JOURNAL OF CURRENT RESEARCH IN MEDICAL SCIENCES - Year 2015, Vol 1, Issue 1
Abstract
BACKGROUND: Plants and plant-based medicaments are the basis of many of the modern pharmaceuticals used for our various ailments ). Progress over the centuries towards a better understanding of a plant derived medicine has depended on two factors that have gone hand in hand. One has been the development of increasingly strict criteria of proof that a medicine really does what it is claimed to do and the other has been the identification by chemical analysis of the active compound in the plant. OBJECTIVE: present study aimed on the In vitro anti-cancer properties of Bryophyllum pinnatum kurz extrac ts by MTT cell line by A 549 (Lung Cancer cell line) and MCF- 7 (Breast cancer cell line) MATERIALS AND METHODS The plant extracts were analyzed for presence of Alkaloids, Flavonoids , Steroids, Terpenoids, Anthroquinones, Phenols, Saponins, Tannins and isolated compound analysed for for the anticancer activitity. RESULTS: The phytochemical investigations of Bryophyllum pinnatum extracts (hexane, ethyl acetate and hydroalcohol) revealed differences in their phytoconstituents and anticancer activity, of cancer cell line A 549. The extract was tested for concentrations of 60.5, 125, 250, 500 and 1000µg/ml. Higher cytotoxicity is seen in 1000µg/ml with a value of 90.1% and CTC50 in µg/ml is 206.CONCLOSION: The highest yield was obtained in hydroalcohol and least yield was found in hexane. The phytochemical investigations of Bryophyllum pinnatum extracts (hydroalcohol, ethyl acetate and hexane) revealed differences in their phytoconstituents. While comparing the phytochemicals present in Bryophyllum pinnatum extracts, hydroalcohol extract found to be more active than other two extract sample. Total antioxidant activity of hydroalcohol extract of Bryophyllum pinnatum, showed that hydroalcohol extract possess high antioxidant activity.
Authors and Affiliations
Supriya Sharma , Shyam Kumar Shiv and Cheluvaraya Kannan Hindumathy*
Keywords
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