In vitro degradation of polycyclic aromatic hydrocarbons by Sphingobium xenophagum, Bacillus pumilus and Pseudomonas plecoglossicida
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 6, Issue 3
Abstract
Polycyclic aromatic hydrocarbons are a class of organic compounds with carcinogenic and genotoxic properties. Biodegradation of such pollutants using microorganisms, especially bacteria, would be a cheap and environmentally safe clean up method. In the present study, a total of 30 anthracene, phenanthrene and pyrene degrading bacteria were isolated from two petroleum contaminated soils in Isfahan-Iran using enrichment technique. Three isolates, showing the highest growth and the lowest pH in their media, were considered as the best hydrocarbon degraders. These isolates were identified to be Sphingobium xenophagum ATAI16, Bacillus pumilus ATAI17 and Pseudomonas plecoglossicida ATAI18 using 16S rDNA gene sequences analyses, and were submitted to GenBank under accession number of KF040087, KF040088 and KF113842, respectively. They were able to degrade 43.31% of phenanthrene, 56.94% of anthracene and 45.32% of pyrene after 9 days, respectively. Strain ATAI18 produced dioxygenase enzyme as an important metabolite for hydrocarbon degradation. The production of biosurfactant was reported positive for both strains of ATAI17 and ATAI18. It was concluded that these three new strains could be used to improve the bioremediation process in a polluted area.
Authors and Affiliations
Imaneh Amini, Arezoo Tahmourespour, Atousa Abdollahi, Mansour Bayat
Keywords
Related Articles
- EP ID EP38352
- DOI -
- Views 155
- Downloads 0
Removal of environmental pollutants azo dye Acid Red 18 in aqueous solution using adsorbent activated carbon of walnut shell
In this paper, removal of Acid Red 18 dye (AR18) from aqueous solutions using activated carbon of walnut shell (ACWS) adsorbent was studied. The prepared ACWS adsorbent was characterized by scanning electron microscopy...
Prediction of the desertification variation trend in Haj Ali Gholi desert basin for 2030 and 2045
Nowadays, prediction of the desertification variation trend used to explain the drought status for the next decades. In this study, using Landsat time series images, desertification variation trend in Haj Ali Gholi basin...
The influence of climate changes on the hydrobionts: a review
Climate and its changes have a direct impact on the development of the different hydrobiont species. These effects on aquatic organisms could be either positive or negative. Every species adapts specifically to natural...
Bioefficacy of botanical extracts and bioagents against sclerotial isolates of Rhizoctonia solani
The antifungal efficacy of six botanical extracts viz., Cannabis sativa L., Peganum harmala L., Datura starmonium L., Artemisia brevifolium L., Capparis spinosa L., Mentha royleana L. and two bioagents viz., Trichoderm...
Rapeseed (Brassica napus L.) genotypes response to NaCl salinity
In order to evaluate the response of rapeseed genotypes to NaCl salinity, a hydroponic experiment at seedling stage with three replications was performed in greenhouse. Twelve spring rapeseed genotypes were treated by so...