In vitro development of embryos from experimentally Kerack-addicted Mice
Journal Title: International Journal of Reproductive BioMedicine - Year 2017, Vol 15, Issue 7
Abstract
Background: Prenatal drug exposure, as a common public health concern, is associated with an increased risk of adverse effects on early embryo development. Objective: To investigate the in vitro development of - embryo from experimentally Kerack-addicted mice. Materials and Methods: Twenty-five female mice were studied in five groups: control, vehicle, and three experimental groups of Kerack-dependent mice (I, II, and III) which received different doses of Kerack for 14 days. After the establishment of addiction model (7 days), experimental groups I, II, and III were given Kerack intraperitoneally at the doses of 5, 35, and 70 mg/kg, twice a day for a period of 7 days, respectively. The vehicle group received normal saline and lemon juice whilst the control group just received water and food. Morulae were obtained through oviduct flashing. The survived embryos were cultured in T6+ 5mg/ml bovine serum albumin. The developmental rates up to hatched stage daily and embryo quality (differential staining and Tunnel staining) were also assessed Results: The developmental potential of embryos obtained from the addicted mother was significantly decreased in comparison with control group. There was a significant reduction in the rate of blastocyst formation in the high dose Kerack dependent group. However, in addicted mice there was reduction in the total cell number (40.92% vs. 65.08% in control) and, inner cell mass percentage (17.17% vs. 26.15% in control) while apoptotic cells numbers were increased (7.17 vs. 1.46 in control) (p<0.05). Conclusion: The Kerack addiction during pregnancy retards preimplantation development and induces apoptosis.
Authors and Affiliations
Elham Mohammadzadeh, Fatemeh-Sadat Amjadi, Mansoureh Movahedin, Zahra Zandieh, Zohreh Nazmara, Neda Eslahi, Peymaneh Shirinbayan, Hamid Reza Asgari, Nahid Azad, Maryam Salimi, Morteza Koruji
Keywords
Related Articles
- EP ID EP245864
- DOI -
- Views 99
- Downloads 0
The association between single nucleotide polymorphism in interleukin-27 gene and recurrent pregnancy loss in Iranian women
Background: Recurrent pregnancy loss (RPL) has been defined as two or more miscarriages before 20th week of gestation. It seems that IL-27 may reduce inflammatory responses and affect the survival of the embryo during hu...
Successful pregnancy and live birth after intrauterine administration of autologous platelet-rich plasma in a woman with recurrent implantation failure: A case report
Background: Platelets contain a significant amount of growth factors that have positive effects on local tissue repair and endometrial receptivity. Case: Here we present a 45-yr-old woman with primary infertility and two...
Association of assisted reproductive technology with adverse pregnancy outcomes
Background: More and more infertile patients have accepted the assisted reproductive technique (ART) therapy. Concerns have been raised over an increased risk of adverse maternal outcomes in ART populations as compared w...
Effects of human menopausal gonadotropin on zona pellucida and pregnancy outcomes of ovarian stimulation protocols
Background: Human menopausal gonadotropin (hMG) has contributed many improvements to human assisted reproduction. However, effects of hMG on oocyte development and clinical results remain controversial. Objective: This s...
Comparison of the effects of Ginger extract with clomiphene citrate on sex hormones in rats with polycystic ovarian syndrome
Background: Polycystic ovarian syndrome (PCOS) is the most common endocrine disorder in women which affect fertility. Clomiphene citrate is used as first-line treatment for this disorder, which is associated with some co...