IN VITRO EVALUATION OF CYTOTOXICITY, OXIDATIVE STRESS, DNA DAMAGE AND INFLAMMATION INDUCED BY DIESEL EXHAUST PARTICLES IN HUMAN A549 LUNG CELLS AND MURINE RAW 264.7 MACROPHAGES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 10
Abstract
Objective: The aim of the present study was to examine the in vitro oxidative stress, cytotoxicity, inflammation and DNA damage induced by Diesel Exhaust Particles (DEPs).Methods: Alveolar macrophages (murine RAW 264.7 cells) and cultured type II epithelium cells (human A549 lung cells) were exposed to various concentrations of Diesel Exhaust Particles for 24 h. The experiment was evaluated for cell viability, oxidative stress, cytotoxicity, inflammation and DNA damage parameters.Results: The results showed that overall both cell lines had similar patterns in response to the oxidative stress, cytotoxicity, inflammation and DNA damage parameters induced by DEPs. Vehicle control showed no changes compared to the control. Both cells showed significant changes at the dose of 20 μg/mL and significant changes were found in cytotoxicity, oxidative stress, DNA damage and inflammation indexes.Conclusion: Hence, exposure to DEPs resulted in doseâ€dependent toxicity in cultured A549 cells and RAW264.7cells and was closely correlated to increased inflammation and oxidative stress.Â
Authors and Affiliations
Durga M, Nathiya. S, Devasena Thiyagarajan
Keywords
Related Articles
- EP ID EP579408
- DOI -
- Views 60
- Downloads 0
CNS DEPRESSANT, SEDATIVE AND ANXIOLYTIC ACTIVITY OF ETHANOLIC EXTRACT OF FRUIT OF PIPER CHABA REVEALED AFTER NEUROPHARMACOLOGICAL SCREENING
Objective: Different ailments can be treated by multiple approaches. Since ancient civilization plant material have been used successfully for treating diseases. Plants contain active ingredients which are beneficial for...
FORMULATION AND EVALUATION OF TABLET PREPARED BY COAMORPHOUS SYSTEM CONTAINING ANTI-HYPERTENSIVE AND ANTI-HYPERLIPIDEMIC DRUG
Objective: Amlodipine besylate (AML) and Atorvastatin calcium (ATR) belong to biopharmaceutical classification system (BCS) class II (i.e. low solubility and high permeability) which leads to variable bioavailability. He...
STUDY ON THE PROPERTIES OF PURIFIED RECOMBINANT SUPEROXIDE DISMUTASE FROM STAPHYLOCOCCUS EQUORUM, A LOCAL ISOLATE FROM INDONESIA
Objective: Superoxide dismutase (SOD) (E. C: 1.15.1.1) from Staphylococcus equorum which catalyzes the dismutation of the superoxide anion (O2.-) into molecular oxygen (O2) and hydrogen peroxide (H2O2), is one of the mos...
PREPARATION AND CHARACTERIZATION OF CURCUMIN LOADED CASSAVA STARCH NANOPARTICLES WITH IMPROVED CELLULAR ABSORPTION
Objectives: The present study describes loading of curcumin onto cassava starch nanoparticles to improve its cellular absorption.Methods: Cassava starch nanoparticles were prepared by acid hydrolysis. A solution of curcu...
TARGETING JATROPHA DERIVED PHYTOCHEMICALS TO INHIBIT THE XANTHINE OXIDASE & CYCLOOXYGENASE-2: IN SILICO ANALYSIS TOWARDS GOUT TREATMENT
Objective: Gouty arthritis is a well known disease with an abrupt attack causing extreme pain in and around the joints. Accumulated urate crystals, being the reason of the disease cause a lot of inflammation leading to s...