In vitro exposure to triclosan regulates hydroxysteroid dehydrogenase activity in epididymal sperm of goat
Journal Title: World Journal of Pharmaceutical Sciences - Year 2016, Vol 4, Issue 4
Abstract
Triclosan, an antibacterial and antimicrobial agent, is widely used in pharmaceuticals, personal care and household products. The present study evaluated the effects of triclosan on sperm functional parameters and steroidogenic enzyme activity in goat epididymal sperm in vitro. Epididymal sperm of mature fertile goat were incubated with triclosan in Ringer’s phosphate solution. Experiments were divided into three groups, such as Group I: Triclosan at different concentrations (50, 100, 200 and 300 μM) for 5 h, Group II and III: Triclosan at 200 and 300 μM concentrations for different durations (1, 2, 3, 4 and 5 h). Epididymal sperm incubated with triclosan showed decline in sperm motility from 77 to 46% and sperm viability from 78% to 47% after 5 h of incubation in concentration-dependent manner. Activities of 3β-HSD decreased significantly at 200 and 300 μM, and 17β-HSD decreased significantly at 100, 200 and 300 μM of triclosan incubation for 5 h. Sperm when incubated with triclosan at 200 and 300 μM concentrations decreased the activities of 3β-HSD and 17β-HSD only after 3 h of incubation. In conclusion, triclosan affects the sperm functions as well as the male fertility by down regulating the activities of testicular steroidogenic enzymes.
Authors and Affiliations
Dr. K. C. Chitra
Keywords
Related Articles
- EP ID EP197613
- DOI -
- Views 75
- Downloads 0
Polyphenols of tea as powerhouse for healthcare because TEA is Tremendous Effective Antioxidant
Tea contains antioxidants. Tea has less caffeine than coffee. Coffee usually has two to three times the caffeine of tea. An eight-ounce cup of coffee contains around 135 mg caffeine; tea contains only 30-40 mg per cup. T...
A Survey on the Knowledge, Attitude, and Practices about anti-biotic resistance among general practitioners of Northern India
The aim of the present study is to conduct a survey on the Knowledge, Attitude, and Practices about antimicrobial resistance among general practitioners of Northern India. This study was a questionnaire based cross secti...
In vitro anthelmintic activity of Sansevieria trifasciata leaves extract against Fasciola hepatica
The purpose of this study was to investigate the in vitro anthelmintic activity of Sansevieria trifasciata leaves extract against Fasciola Hepatica. The extract was prepared by soaking one portion of the plant leaves int...
Optimization of Microwave Assisted Extraction of Total Phenolic Contents from Triticum aestivum L. Whole-grain Cereal
Whole-grain cereals have unique blend of bioactive constituents and antioxidants which are proposed to be responsible for the health profits of whole-grain consumption. Nevertheless, phytochemicals and antioxidants in wh...
Effectiveness of Attai Vidal (leech therapy) on Naala Vibatha Karappan (stasis eczema)
Siddha Medicine is unique system of medicine in the Indian subcontinent and some other Asian countries of the globe. Based on Siddha medicine, diseases are classified in to 4448. Ailments could be treated by Internal med...