In Vitro Fertilization in Sheep Using Long Standing Capacitated Spermatozoa (Abattoir sample)
Journal Title: PARIPEX-Indian Journal of Research - Year 2016, Vol 5, Issue 5
Abstract
Abattoir fresh genetalia could be regarded as a highly yielding male (spermatozoa) and female (oocyte) samples, in which these samples if prepared well considered as good reservoir for male and female gametes than could be contributed to the advanced reproductive technique (ART). Fresh female and male whole genitalia had been collected from Al-Shoáalla abattoir, west of Baghdad on January 2014 to March 2015, collected samples transported by cool box (4-8℃) directly to Lab. Sample washed thoroughly with distilled water, ovaries dissected and separated from surrounding tissue, washed with d. water containing antibiotic and antifungal preparation, ovaries bearing large follicles (3-6mm) was inspired by 18 gauge needle, entire ovaries then sliced by scalpel blade to small pieces. Collected oocytes incubated for IVM and IVF. Collected methods showed; 380 oocytes had been collected by aspiration method toward 1570 oocytes in slicing method with a degree of maturation 223 to 752 Oocytes for the aspiration and slicing methods respectively. Seasonality affect the grading and fertilization index (P<0.05), good grade showed 364 to 878 (out to within season respectively) toward fair grade 104- 252 (out to within season respectively) while fertilization percentage showed 49.55% out of season toward 57.42% within season. Cauda samples were sliced and incubated at (RT) for following period of time capacitated with heparin gave a good fertilizable ability of these spermatozoa even for this prolonged period of time.
Authors and Affiliations
Wafer M. Saleh, Ihsan H. AL-TIMIMI
Keywords
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