In Vitro Flower Bud Formation, Plant Regeneration and Morphogenetic Studies in Local Scented Cultivar of Rosa indica
Journal Title: Journal of Ornamental and Horticultural Plants - Year 2014, Vol 4, Issue 1
Abstract
In vitro propagation of rose (Rosa indica) as well as rose breeding is a valuable economic enterprise and has received highest importance globally including Indian subcontinent. The development of new cultivars for commerce is faced with new challenges. In vitro micropropagation protocols of varieties of roses have been established but very few reports on the clonal propagation of local cultivars of Indian scented rose has been made. In the present paper we report the dedifferentiation and redifferentiation of stem nodal, leaf disc, petals well as ovary explants cultured on MS medium supplemented with various growth adjuvants such as coconut water (CW 5%-20% v/v), auxins (IAA, NAA, 2, 4-D and IBA) and cytokinins (BAP and Kn) in varying concentrations. Vigorous callus formation was observed showing embryogenic clusters in both the nodal and leaf disc explants on MS medium supplemented with CW (10% v/v) +2, 4- D (11.3 mM) +BAP (3.55 mM). The proliferation of 4-7 multiple shoots (mean number of shoots 5±1.24) as well as in vitro flower bud formation on in vitro regenerated rose plants from nodal explants cultured on MS medium supplemented with CW+2, 4-D+BAP have been observed. The regenerated shoots treated with ‘Quic Root’ (commercially available rooting chemical) produced well developed root system.
Authors and Affiliations
Raageeva Bimal, Niti Kiran
Keywords
Related Articles
- EP ID EP138476
- DOI -
- Views 104
- Downloads 0
[b]An Easy and Simple Method for Estimating Total Shoot Length During Screening and Evaluation of Mulberry ([i]Morus spp[/i].) Genotypes[/b]
In mulberry (Morus sp.), grown for its foliage, which is the sole food for the silkworm (Bombyx mori L.), evolving high yielding varieties is a long drawn and laborious process. One of the important selec...
[b]Influence of Different Colored Plastic Mulch on the Growth of Lettuce ([i]Lactuca sativa[/i]) [/b]
Mulches bring several benefits to lettuce cultivation.A study on the lettuce ‘Red Rapids’ has been conducted to determine the influence of the different colored plastic mulch on its growth. The experiment consisted of fi...
[b] Application Methods of Thymus vulgaris Essential Oil and Their Effect on Vase Life and Qualitative Traits of Gladiolus grandiflorus L. Cut Flowers[/b]
In this study, combined effect of two essential oils, Thymol and Carvacrol, with different concentrations in two methods on Gladiolous grandiflorous L. was investigated based on a completely randomized de...
[b] An Efficient and Cost Effective Protocol for In Vitro Propagation of Pineapple[/b]
An efficient and cost effective protocol for in vitro propagation of Pineapple (Ananas comosus var. Queen) has been developed. In the proliferation stage, agar based Murashige and Skoog (MS) media was su...