In-Vitro Hydrogen Peroxide Scavenging Activity of Ethanol Extract of Canthium coromandelicum
Journal Title: PARIPEX-Indian Journal of Research - Year 2016, Vol 5, Issue 2
Abstract
INTRODUCTION:Canthium coromandelicum is a shrub widely indicated for dysentery. An antioxidant is molecule that inhibits the oxidation of other molecules. Antioxidants are widely used in dietary supplements and have been investigated for the prevention of diseases such as cancer, coronary heart disease and altitude sickness. In this study we have attempted to evaluate the free radical scavenging activity of canthium coromandelicum using in-vitro hydrogen peroxide. OBJECTIVE:The objective of the present study was to evaluate the free radical scavenging activity and antioxidant potential of Canthium coromandelicum. MATERIAL & METHODS:Hydrogen peroxide scavenging activity was determined according to the method of Ruch et al (1989). The principle of this method is that there is a decrease in absorbance of H2O2 upon oxidation of H2O2. Hydrogen peroxide solution (40mM) was prepared in phosphate buffer (pH 7.4). Canthium coromandelicum extract (100 μg/mL) in distilled water was added to hydrogen peroxide solution (0.6 mL, 40mM). Absorbance of hydrogen peroxide at 540 nm was determined 10 minutes later against a blank solution containing the phosphate buffer without hydrogen peroxide. The resultant absorbance was recorded at 540 nm using spectrophotometer. The percentage inhibition was calculated using the formula Percentage inhibition = Abs control- Abs sample * 100 Abs control RESULTS:At concentrations of 20, 40, 60, 80 μg/ml percentage of inhibition observed was 10.68±0.10, 20.39±0.37, 43.56±0.09 and 74.03±0.67 respectively. DISCUSSION & CONCLUSION:Thus this invitro study suggest that Canthium coromandelicum possesses antioxidant properties. Prior phytochemical analysis Canthium coromandelicum shows the presence of flavonoids, tannins and glycosides (Mohan et.al). The antioxidant property of Canthium coromandelicum may be attributed to the presence of flavonoids and tannins. However pharmacokinetic and safety profile of Canthium coromandelicum requires pre-clinical testing prior to its clinical application in human beings.
Authors and Affiliations
Dr. Sonali Kirde, Dr. V. P . Karthik, Dr. Sushil kumar Varma
Keywords
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