In vitro Investigation of Hydro-alcoholic Extract of Helichrysum leucocephalum on the Inhibition of Streptococcus Mutans Growth
Journal Title: Journal of Dental Biomaterials - Year 2017, Vol 4, Issue 4
Abstract
Statement of Problem: Indiscriminate use of antibacterial agents increases the antibiotic resistance, which consequently necessitates seeking alternatives such as herbal remedies. Objectives: The aim of this study was to evaluate the antimicrobial effect of hydroalcoholic extract of Helichrysum leucocephalum (H.leucocephalum) on Streptococcus mutans (S. mutans) growth as a major cause of dental caries. Materials and Methods: In this study, hydroalcoholic extract of H. leucocephalum was prepared. The antibacterial effects, minimal inhibitory concentration (MIC), and minimal bactericidal concentration (MBC) of hydroalcoholic extract of H. leucocephalum and penicillin were assessed. Agar well diffusion method and micro broth dilution assay were used on bacterial suspension adjusted to a 0.5 McFarland standard (equivalent to 1.5×108 CFU/mL).Each test was repeated four times to minimize lab errors. Results: In this study, the inhibitory zone of hydroalcoholic extract of H.leucocephalum in concentration of 100 mg/mL was 34±0.1 mm and for penicillin was 10 mm. The MIC and MBC of H.leucocephalum hydroalcoholic extract were 5.6±6.25, 21.6±6.25 mg/mL, respectively. Conclusions: Hydroalcoholic extract of H. leucocephalum have antibacterial effect on S.mutans at a concentration of 12.5mg/mL, indicating possible application of this extract in prevention of dental caries; however, future researches are necessary.
Authors and Affiliations
Mohammad Motamedifar, Ali Nozari, Elmira Azhdari Ghasrodashti
Keywords
Related Articles
- EP ID EP248699
- DOI -
- Views 53
- Downloads 0
Comparison of Mechanical Properties of Resin Composites with Resin Modified Glass Ionomers
Statement of Problem: There are controversial reports regarding physical and mechanical properties of resin composites and glass ionomer cements. Some revealed higher strength and hardness for resin composites while othe...
Evaluation of Surface Characteristics of Denture Base Using Organic-Inorganic Hybrid Coating: An SEM Study
Statement of Problem: Despite the numerous positive features of acrylic denture base, there are a number of undeniable associated disadvantages. The properties of denture base have been improved through various intervent...
An In vitro Analysis of the Effects of Iron Sulfate and Iron Acetate on Streptococcus mutans
Statement of problem: Dental caries is a common infectious disease induced by Streptococcus mutans (S. mutans). Objectives: Due to the high incidence rate of dental caries and iron deficiency in the Iranian population,...
Chemical Stability of Bioglass in Simulated Oral Environment
Statement of Problem: Bioglasses are a series of biocompatible dental materials, which are considered as light conducting inserts in resin composite restorations. Consequently, their chemical stability is more essential...
Cytokeratin: a Shortcut to Diagnose Spindle Cell Carcinoma
A relatively rare subtype of squamous cell carcinoma (SCC) is spindle cell carcinoma (SPCC). It is composed of epithelium-derived spindle cells arranged in sheets with mesenchymal properties and small, hard-ly detectable...