IN VITRO MASS PROPAGATION OF GYNURA PROCUMBENS (LOUR.) MERR. - AN IMPORTANT MEDICINAL PLANT
Journal Title: ASIAN JOURNAL OF NATURAL & APPLIED SCIENCES - Year 2016, Vol 5, Issue 3
Abstract
An improved protocol was developed for direct micropropagation of Gynura procumbens (Lour.) Merr-an important medicinal plant, from different explants (shoot tips and nodal segments) in Murashige and shoog (MS) basal medium supplemented with different growth regulators like, Cytokinins and Auxins either alone or in combinations. Highest rate (100%) of shoot induction was observed on MS medium supplemented with 1.5 mg/l 6-benzylaminopurine (BAP) and 1.0 mg/l α-naphtalene acetic Acid (NAA) from nodal explants. The highest elongation of shoot buds (8.36 cm) was obtained on MS basal medium fortified with 1.0mg/l 6-benzylaminopurine (BAP) and 0.25 mg/l α-naphtalene acetic acid (NAA). The maximum number of roots (6.91±0.50) and root length (7.60cm) was observed in half strength MS medium supplemented with 0.5 mg/l indole-3-butyric acid(IBA).The rooted plantlets were acclimatized and transferred to greenhouse with 97% success. This in vitro propagation protocol should be useful for conservation as well as mass propagation of this medicinal plant.
Authors and Affiliations
Sweety Majumder, Animesh Biswas, Md. Mahbubur Rahman
Keywords
Related Articles
- EP ID EP204467
- DOI -
- Views 99
- Downloads 0
RENEWABLE ENERGY AND RENEWABLE R&D IN EU COUNTRIES: A COINTEGRATION ANALYSIS
In recent years, increased attention has been directed towards carbon dioxide emissions and environmental impacts, as industrialized countries have had to find ways of reducing energy use in order to meet Kyoto t...
SOIL MICROBIAL COMMUNITIES AND ENZYME ACTIVITIES UNDER DIFFERENT CROPPING SYSTEMS
Current trend in the global agriculture is to search for highly productive; sustainable and ecologically friendly cropping systems. Studies have been disclosed that some cropping systems had impacts on the microorganisms...
CELL PHONES ARE MORE DANGEROUS THAN SMOKING
The topic of this paper is that cell phones are dangerous like smoking. This comparison between cell phone and smoking used to show how much cell phone usage can be dangerous on human life. The main four parts of this pa...
IONOSPHERIC ANOMALY FROM HUGE GAS EXPLOSION IN KAOHSIUNG CITY IN TAIWAN ON 31 JULY 2014
This paper used two-dimensional Principal Component Analysis (2DPCA) to determine total electron content (TEC) respond in the ionosphere for the huge gas explosion in Kaohsiung, Taiwan at 15: 59 (UT) on 31 July 2014. Res...
IMPACT OF FEEDING SELECTED MULBERRY GERMPLASM VARIETIES ON SILKWORM BOMBYX MORI L. THROUGH BIOASSAY TECHNIQUES FOR COMMERCIAL EXPLOITATION
A critical evaluation study comprising five mulberry varieties viz., S1708, MS5, C10, C6 and M5 was conducted for their leaf quality through silkworm moulting and rearing using crossbreed (PMxNB4D2) silkworms. Results re...