IN-VITRO MORPHOGENESIS STUDIES IN Gerbera jamesonii BOLUS EX HOOKER F
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 52
Abstract
During present investigation, mature embryos were used as explant sources and cultured on MS medium amended with diverse auxins and cytokinins in varying concentrations as sole as well as in different combinations Culture media MS3D.5B (MS + 3.0 mg. l-1 2, 4 D + 0.5 mgl-1BA + 30.0 gl-1 sucrose + 7.5 gl-1 agar powder) proved well for callus initiation. Culture media MS2N.5iP/MS3N.5ip (MS + 2.0/3.0mg l-1 NAA + 0.5 mgl-12-ip + 30.0 gl-1 sucrose + 7.5 gl-1 agar) exhibited higher response i.e. number of shoot proliferating explants and number of shoot (s) per explant. However, shoot of higher length was recorded on nutrient medium MSB/MS2B (MS + 2.0/3.0 mgl-1 BA + 30.0 gl-1 sucrose + 7.5 g l-1 agar). Higher rooting response (root proliferating efficiency, number of roots and mean root length) was exhibited by rooting medium MS.1IB (MS + 0.1 mgl-1IBA + 15.0gl-1 sucrose + 7.5 g l-1 agar). The plantlets were transferred to pots and hardened in Environmental Growth Cabinet and Net House during initial weaning period and transferred to field successfully. Phenotypic normal plants were recovered.
Authors and Affiliations
R. JATAV
Keywords
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