In vitro multiplication of the endangered plant species, Exacum bicolor Roxb
Journal Title: Journal of Research in Biology - Year 2016, Vol 6, Issue 5
Abstract
To conserve endangered medicinal plant species, Exacum bicolor Roxb. (Gentianaceae), an efficient micropropagation protocol was developed by using nodal segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be successfully acclimatized at a rate of 80 % survivability
Authors and Affiliations
Jeeshna MV
Keywords
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