In vitro of the fixed oil of pinhão bravo on control of Colletotrichum musae
Journal Title: Revista Verde de Agroecologia e Desenvolvimento Sustentável - Year 2019, Vol 14, Issue 2
Abstract
The anthracnose caused by the fungus Colletotrichum musae is considered the most important diseases in banana fruits. The objective of this work was to evaluate the effect of the fixed oil of pinhão bravo in the in vitro control of C. musae, in different concentrations and application methodologies. The fungus was isolated from banana fruits, grown in Potato-Dextrose-Agar (B.D.A) culture medium. The experiment was conducted in a factorial scheme with 2 factors (3 application methods and 3 concentrations + 2 controls), with seven replicates. The methods of application were: incorporation of the oil into the fuse culture medium; distribution of the oil in the solidified culture medium and distribution of the oil in filter paper, fixed to the inner part of the lid of the Petri dish. The concentrations of the oil used were: 0.1; 1 and 10%. The controls were: absolute control (without oil and without fungicide) and relative control (without oil and with fungicide). The oil tested at all concentrations and application methods was diluted in Tween 80. The radial growth in centimeter of the mycelium of the fungus was measured daily for ten days. The data were analyzed by the Tukey test at 5% probability. The Treatments 1, 2, 5, 7, 8 and 11 provided greater inhibition of the mycelial growth of C. musae and were statistically equal to each other. The fixed oil of pinhão bravo can be used as alternative measure of control of the C. musae in substitution to the chemical pesticides.
Authors and Affiliations
Frank Magno da Costa, Izaías Araújo de Oliveira, Mayara Fernandes dos Santos
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