Abstract

Rheum webbianum commonly known as œrhubarb is a reputed medicinal plant belonging to family Polygonaceae. An attempt has been made to carry out the in vitro regeneration of this important medicinal plant species using hypocotyl segments as explants. Direct organogenesis occurred when the hypocotyl segments from the in vitro germinated seedlings were inoculated on MS medium containing different growth regulators at various concentrations and combinations. Cytokinins like BAP and Kn were found to be effective in shoot regeneration both individually as well as in combination with auxins like IAA, IBA and 2,4-D MS medium supplemented with BAP at a concentration of 2mg/l in combination with IAA at a concentration of 3mg/l was found to be the best medium for shoot regeneration. For rooting of regenerated shoots half strength MS medium supplemented with 0.5mg/l IAA was found to be most effective. Sabreena Rashid | Zahoor A Kaloo | Seema Singh | Iram Bashir"In vitro plant regeneration from hypocotyl explants of Rheum webbianum Royle" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-2 , February 2018, URL: http://www.ijtsrd.com/papers/ijtsrd9558.pdf http://www.ijtsrd.com/biological-science/botany/9558/in-vitro-plant-regeneration-from-hypocotyl-explants-of-rheum-webbianum-royle/sabreena-rashid

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