IN VITRO PLASMID CURING ACTIVITY OF AQUEOUS EXTRACT OF TERMINALIA CHEBULA FRUIT AGAINST PLASMIDS OF BACILLUS SUBTILIS AND SHIGELLA SONNEI
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 4
Abstract
Objective: The aim of this investigation was to evaluate in vitro plasmid curing activity of the aqueous extract from Terminalia chebula fruit against multi-drug resistance bacteria.Methods: The aqueous extract of Terminalia chebula fruit was prepared in citrate phosphate buffer, pH 6.8. The extract was partially purified through gel chromatography. Antibacterial and antiplasmid activity against Salmonella typhi, Bacillus subtilis, Escherichia coli and Shigella sonnei was checked using the broth dilution method. The contents of crude extract were estimated.Results: The crude extracts exhibited anti plasmid activity against plasmids of B. subtilis and Sh. sonnei. Loss of plasmid band was confirmed by agarose gel electrophoresis. The curing efficiency of aqueous extract was found to be 100% and 44% against plasmids of B. subtilis (pUB110) and Sh. sonnei(pARI-815) respectively. The results were compared with conventional curing agent such as ethidium bromide and acridine.Conclusion: The aqueous extract of T. chebula fruit has the potential to inhibit growth of strains which are resistant to many antibiotics. Results could provide new insight into the treatment of infectious diseases which are facing limitation of antibiotics.Â
Authors and Affiliations
Pratibha Srivastava, Renuka S. Wagh, Ninad V. Puranik, Hemalata M. Puntambekar, Sheetal S. Jahagirdar, Prashant K. Dhakephalkar
Keywords
Related Articles
- EP ID EP578889
- DOI -
- Views 69
- Downloads 0
RARE SURVIVAL AFTER SEVERE ALUMINIUM PHOSPHIDEPOISOININGAFTER MYOCARDITISWITH L-CARNITINE AND STEROID
Acute Aluminium Phosphide or Celphos poisoning is a common agrochemical fumigant used commonly in countries like South East Asia and China. It is extremely lethal poison as there is no specific antidote and involvement o...
DESIGN ZOLMITRIPTAN LIQUISOLID ORODISPERSIBLE TABLETS AND THEIR IN VITRO EVALUATION
Objective: The objective of present study is to develop orodispersible tablets (ODTs) of zolmitriptan by liquisolid technique using different types of super disintegrants to enhance the disintegration and dissolution of...
EVALUATION OF ANTI-RENOTOXIC RESPONSES IN FLOWERS OF INDIAN LOTUS (NELUMBO NUCIFERA GAERTN.)
Objective: The present study was formulated in order to evaluate anti-renotoxic responses in flowers of an Indian lotus (Nelumbo nucifera Gaertn.)Methods: Gentamicin at a dose of 100 mg/kg/day was used intra-peritonealy...
PREVALENCE OF MALARIA CASES IN TEA GARDEN AREAS OF LAKHIMPUR DISTRICT, ASSAM
Objective: After observing the increasing trend in numbers of fever cases from Joyhing and Koilamari tea estate, mass fever surveys were conducted to determine the prevalence of malaria cases in the affected areas.Method...
FORMULATION AND EVALUATION OF GARLIC POWDER LOADED FLOATING MATRIX TABLET
Objective: The objective of the present work was to formulate and evaluate a stable, odour free garlic powder loaded floating matrix tablet for the treatment of peptic ulcers. Methods: A gastro-retentive floating matrix...