In vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834
Journal Title: Journal of Herbal Drugs - Year 2017, Vol 8, Issue 1
Abstract
Background & Aim: A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying the production of alkaloid in culture. Experimental: The Atropa komarovii leaves were wounded and infected with soil gram-negative bacterium Agrobacterium rhizogenes ATCC15834. After three weeks, the transformation roots and control roots without infection, appeared, and for confirming that T-DNA Ri plasmid fragments were transformed and integrated to plant genome, the rolB gene region, was amplified using PCR. HPLC method was then used for assaying how two tropane alkaloids such as atropine (hyosciamine) and scopolamine (hyoscine) were produced in hairy roots,control roots, leaves and roots of plantlet. Results: The data indicated that diagnostic 500bp rol B product amplification was exhibited to be present by all the transformed hairy roots. Scopolamine content in hairy roots was considerably greater than that in control roots but greatest (Hyoscyamine) atropine content was observed in control roots. Analysis of DW, FW and root length showed that fresh and dry root weight increased in hairy roots compared with that in non transformed root. Recommended applications/industries: The present study demonstrated that secondary metabolite production using medicinal plants concerns many researchers worldwide today and hairy root culture is a useful method for producing tropane alkaloids in solanaceae.
Authors and Affiliations
Ofelia Banihashemi; Ramazan-Ali Khavari-Nejad; Narguess Yassa; Farzaneh Najafi
Keywords
Related Articles
- EP ID EP23540
- DOI -
- Views 326
- Downloads 17
Optimisation of ultrasound-assisted extraction of phenolic compounds from Flomidoschema parviflora
Background & Aim: Restrictions on the use of synthetic antioxidant such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) are being imposed because of their carcinogecity . Experimental: In this invest...
Identification of phytochemicals, plant compounds Smirnovia iranica.
Background & Aim: Fabaceae plant family cow tail (Smirnovia iranica) is one of the valuable species of native shrubs and sand dunes in the central region of Iran which is consistent with the production of forage and soil...
Chemical composition and antimicrobial activity of Achillea wilhelmsii C.Koch essential oil against selected bacterial and fungal pathogens of fish
Background & Aim: Increasing resistance of fish pathogens to chemical drugs, using herbal plants with antimicrobial properties is necessary. In this study, the chemical composition and antimicrobial activity against b...
Genetic improvement of essence percent and dry flower yield using indirect selection in German chamomile (Matricaria chamomilla L.)
Background & Aim: German chamomile is one of the most important medicinal plants. So, assessment of genetic diversity and relationship among the traits are very essential in chamomile breeding programs. Determination of...
Anti-inflammatory activity of leaf extract of Solanum anomalum
Background & Aim: Solanum anomalum Thonn. ex Schumach. (family Solanaceae) is a shrub growing up to 2 metres tall. The edible fruits are gathered from the wild and consumed locally and the leaves are used locally in t...