In vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834
Journal Title: Journal of Herbal Drugs - Year 2017, Vol 8, Issue 1
Abstract
Background & Aim: A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying the production of alkaloid in culture. Experimental: The Atropa komarovii leaves were wounded and infected with soil gram-negative bacterium Agrobacterium rhizogenes ATCC15834. After three weeks, the transformation roots and control roots without infection, appeared, and for confirming that T-DNA Ri plasmid fragments were transformed and integrated to plant genome, the rolB gene region, was amplified using PCR. HPLC method was then used for assaying how two tropane alkaloids such as atropine (hyosciamine) and scopolamine (hyoscine) were produced in hairy roots,control roots, leaves and roots of plantlet. Results: The data indicated that diagnostic 500bp rol B product amplification was exhibited to be present by all the transformed hairy roots. Scopolamine content in hairy roots was considerably greater than that in control roots but greatest (Hyoscyamine) atropine content was observed in control roots. Analysis of DW, FW and root length showed that fresh and dry root weight increased in hairy roots compared with that in non transformed root. Recommended applications/industries: The present study demonstrated that secondary metabolite production using medicinal plants concerns many researchers worldwide today and hairy root culture is a useful method for producing tropane alkaloids in solanaceae.
Authors and Affiliations
Ofelia Banihashemi; Ramazan-Ali Khavari-Nejad; Narguess Yassa; Farzaneh Najafi
Regulation of Intestinal GLP-1 and GLUT2 genes underlie hypoglycemia in Desplatsia subericarpa (Bocq)-Fed Wistar Rats
Background & Aim: Indigenous people of West Africa use the wholeleaf of Desplatsia subericarpa (Bocq) in anti-diabetic soup delicacy. This study was designed to validate the anti-diabetic claims and delineating possibl...
Investigation of main constituents of Satureja hortensis L. essential oil under sowing date and plant density in Mazandaran province
Background and aim: The genus Satureja L. (Lamiaceae) includes 12 species of annual and perennial herbaceous plant in Iran. Satureja species have economic and medicinal importance because of their high essential oil cont...
Anti-ulcerogenic activity of stem extract and fractions of Homalium letestui
Background & Aim: Homalium letestui Pellegr (Flacourtiaceae) used traditionally by the Ibibios of Southern Nigeria to treat stomach ulcer, malaria and other inflammatory diseases was evaluated for antiulcer properties...
Effect of Morus alba leaf extract on serum glucose and lipids in diabetic rats
Background & Aim: Diabetes mellitus is a chronic disease characterized by elevated blood glucose levels and disturbances in carbohydrate, fat and protein metabolism. The objective of the present investigation was to eval...
Antimicrobial Effects of Zataria multiflora Essential Oils on Acinetobacter Strains Isolated from Clinical Specimens
Background & Aim: The aim of this study was to investigate the antimicrobial effects of Zataria multiflora against some Acinetobacter baumannii strains isolated from clinical samples. Experimental: Twelve strains of A...