In Vitro Propagation of Alternanthera sessilis L. from Internode Explant
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 1
Abstract
An efficient protocol was developed for micropropagation of Alternanthera sessilis L. from internode explant. Callus was developed from the cut ends and surface of the internode explants when the Murashige and Skoog (MS) medium was supplemented with 0.5mg/L or 1.0mg/L 2,4-D. The greatest mean number of shoots (124) with the highest mean shoot length (9.3cm) was obtained when calli were cultured on the MS medium with 1.0mg/L each of 6-Benzylaminopurine and adenine sulfate. Early and maximum root induction was noted when shoots were cultured in half strength MS basal medium. In vitro regenerated plants were successfully hardened in a mixture of soil and cow dung in ratio of 3:1 and transferred to field. Cent percent plants survived in field condition, morphologically identical to parent plant and showed normal flowering.
Authors and Affiliations
Dimpy Das, P. K. Borua
Keywords
Related Articles
- EP ID EP237177
- DOI 10.9734/BBJ/2014/6261
- Views 157
- Downloads 0
Towards Efficient In vitro Regeneration of Cowpea (Vigna unguiculata L. Walp): A Review
Cowpea is a crop of tremendous economic and ecological values particularly in sub-Saharan Africa, where over 80% of the crop is produced and consumed. Due to heavy attack by pests and diseases, actual yield does not exce...
Development of Maize Haploid Inducer Lines and Doubled Haploid Lines in Pakistan
The work on maize doubled haploid development has started at the Maize Research Station, Ayub Agricultural Research Institute, Faisalabad, Pakistan during 2010 in collaboration with University of Agriculture Faisalabad,...
Treatment of Wastewaters Generated by Surfactant-Enhanced Washing of Soils in an Aerobic Biofilter Inoculated with a Consortium of Hydrocarbon Degraders
Aims: The aims of this work were 1) To evaluate the performance of a submerged biofiltration system for the treatment of a surfactant-enriched wastewater that had been generated by a soil washing process. 2) To evaluate...
Influence of Type and Age of Primary Somatic Embryo on Secondary and Cyclic Somatic Embryogenesis of Cassava (Manihot esculenta Crantz)
This study investigated the influence of age of the cotyledons, cut from primary somatic embryos (PSE) developed from shoot meristems (SM) or immature leaf lobes (LL), on secondary somatic (SSE) and cyclic (CSE) embryoge...
Production and Evaluation of Biosurfactant by Serratia marcescens UCP 1549 Using Industrial Wastes
Aims: Biosurfactants are surface-active agents of microbial origin that have a property of lowering the surface tension between two liquids. This study aimed to the production of biosurfactant by Serratia marcescens UCP...