In vitro propagation of Hylocereus monacanthus (Lem.) Britton and Rose
Journal Title: Biotecnología Vegetal - Year 2016, Vol 16, Issue 2
Abstract
The application of tissue culture contributes to the rapid and massive propagation of economically important species and serves as a basic platform for production strategies. The objective of this work was to establish the in vitro propagation of Hylocereus monacanthus (Lem.) Britton and Rose. The seeds were in vitro germinated and then the apical segments containing the apex and areoles were taken and placed in culture medium MS with BAP (1.0, 2.0 and 4.0 mg l -1 ) and IAA (0.5 mg l -1 ) separately and combined for the multiplication phase. For rooting an MS culture medium with different concentrations of inorganic salts (50, 75 and 100%) and IBA (0.1 mg l -1 ) was used. In vitro plants obtained were planted in greenhouse for their acclimatization. The percentage of seeds germination was 70% with 6% of microbial contamination. With 1 mg l -1 BAP, the best results were obtained for the in vitro multiplication of H. monacanthus. In all treatments 100% of rooted shoots were obtained and only a significant difference was observed for root length with the addition of 0.1 mg l -1 IBA. The average survival of plants transferred to substrate was 97.1%. The results of this work offer an alternative of propagation for H. monacanthus, which will contribute to the establishment of commercial plantations and other studies at the laboratory level.
Authors and Affiliations
Laura Belem Montiel-Frausto , José Raymundo Enríquez del Valle , Aroldo Cisneros
Keywords
Related Articles
- EP ID EP683061
- DOI -
- Views 148
- Downloads 1
New explant for somatic embryogenesis induction and plant regeneration from diploid banana (‘Calcutta 4’, Musa AA)
A m et hod has b een devel oped f or plant regeneration vi a em bry ogenic c ell suspensions from dipl oi d cult ivar ‘Cal cutt a 4 ́. For callus induction with embryogenic structures, different plant tissues such as sc...
Quantification of Mycosphaerella fijiensis in vitro growth by absorbance readings
The slow growth of Mycosphaerella fijiensis Morelet in synthetic and semi-synthetic media as well as the characteristics of their colonies makes the use of traditional methods for evaluating the fungal growth are inade...
Establishment of sugarcane mother plant bank in semicontroled conditions for in vitro propagation
The propagation of sugarcane (Saccharum spp.) Requires seed with physiological and phytosanitary quality. The work was carried out with the objective of establishing a bank of sugarcane mother plants under semi-controlle...
Effect of two plant growth regulators and illumination conditions in the germination of conserved seeds of Clitoria ternatea
The seeds viability lost in the seed legume bank of Research Institute of Pastures and Forages (IIPF) led to the aim of the work it was to determine the effect of two plant growth regulators (gibberellic acid; GA 3 and n...
Effect of LED light on seeds of Capsicum annuum L. var. serrano
Serrano pepper (Capsicum annuum L.) is a crop of economic, nutritional and medicinal importance; so it is necessary to increase its production and improve its characteristics. The objective of this research was to eval...