In vitro propagation of Hylocereus monacanthus (Lem.) Britton and Rose
Journal Title: Biotecnología Vegetal - Year 2016, Vol 16, Issue 2
Abstract
The application of tissue culture contributes to the rapid and massive propagation of economically important species and serves as a basic platform for production strategies. The objective of this work was to establish the in vitro propagation of Hylocereus monacanthus (Lem.) Britton and Rose. The seeds were in vitro germinated and then the apical segments containing the apex and areoles were taken and placed in culture medium MS with BAP (1.0, 2.0 and 4.0 mg l -1 ) and IAA (0.5 mg l -1 ) separately and combined for the multiplication phase. For rooting an MS culture medium with different concentrations of inorganic salts (50, 75 and 100%) and IBA (0.1 mg l -1 ) was used. In vitro plants obtained were planted in greenhouse for their acclimatization. The percentage of seeds germination was 70% with 6% of microbial contamination. With 1 mg l -1 BAP, the best results were obtained for the in vitro multiplication of H. monacanthus. In all treatments 100% of rooted shoots were obtained and only a significant difference was observed for root length with the addition of 0.1 mg l -1 IBA. The average survival of plants transferred to substrate was 97.1%. The results of this work offer an alternative of propagation for H. monacanthus, which will contribute to the establishment of commercial plantations and other studies at the laboratory level.
Authors and Affiliations
Laura Belem Montiel-Frausto , José Raymundo Enríquez del Valle , Aroldo Cisneros
Keywords
Related Articles
- EP ID EP683061
- DOI -
- Views 154
- Downloads 1
Evaluation of indicators for the early selection of the height character in Musa spp.
In the plant breeding programs attended by Biotechnology and using the induction of mutations becomes necessary to work with big populations of plants to guarantee bigger possibilities to be successful in the work. For w...
Selection of Azotobacter chroococcum strains for its application on in vitro pineapple cv. ‘Cayena lisa’ plants acclimatization
Azotobacter chroococcum is a rhizosphere bacterium that has been used for decades as a plant growth stimulator in a wide range of crops. It is able to fix nitrogen and produce growth promoting substances. In the presen...
Use of biotechnological methods in the potato seed production
Potato crop has a large economic importance. Worldwide, propagation of potato by in vitro culture of axillary buds is commonly used in the production of in vitro plants and microtubers. These constitute the core plant ma...
Influence of 6-Benzylaminopurine on the in vitro behavior of Henequen plants obtained from embryos
In the acclimatization phase, a group of derived plants from henequen embryos, germinated in a medium with different concentrations of 6 BAP, was evaluated. In these results it is evidenced that the content of 6 BAP in t...
Foliar anatomy and morphological characterization of Bambusa vulgaris in the acclimatization phase
This study was aimed to achieve the morphological and anatomical characterization of in vitro plants of Bambusa vulgaris Schrad. ex Wendl during acclimatization. The morphological development of the leaf and root in pl...