In Vitro Propagation of Ornothogalum umbellatum
Journal Title: ISPEC Journal of Agricultural Sciences - Year 2023, Vol 7, Issue 4
Abstract
The objective of this study is to determine the propagation performance of Ornithogalum umbellatum, which is among the geophytes of our country, by tissue culture. The study was conducted in the tissue culture laboratory of the Field Crops Department of Agricultural Faculty of the Ege University. Bulbs explants of Ornithogalum umbellatum were used as genetic material in the study. Bulb explants were cultured in different cytokinins for the rejeneration in vitro conditions. The media of different levels of MS+Z (Zeatin) (0.5; 1.0 ve 2.0 mg L-1), MS +TDZ (Thidazuron) (3.0 ve 4.0 mg L-1) ve MS+BAP (6-Benzilaminopurin) (4.0 mg L-1) were used for in vitro propagation. There was no difference in media of Z, TDZ and BAP for the number of shoots. The medium of 0.5 m L-1 Z had the highest mean in terms of shoot length as 10.2 cm. For root number and root length were found to be successful in 0.5 mg L-1 and 1.0 mg L-1 Z but root formation was not achieved in the other media. Developing shoots were sub cultured in different amounts of IBA for root formation. While there was no difference in the IBA media for root number, 0.5 mg L-1 and 1.0 mg L-1 IBA had the highest mean in terms of root length. Bulbs explants of Ornithogalum umbellatum were found to be suitable for propagation under in vitro. Therefore, it can be used in a rapid and disease-free plantlet by in vitro culture and can be evaluated in various area, just as commercial production.
Authors and Affiliations
Gülsüm ÖZTÜRK
Keywords
Related Articles
- EP ID EP724747
- DOI https://doi.org/10.5281/zenodo.10225471
- Views 35
- Downloads 0
Chicken Meat Consumption Habits and Preferences of a State University Van Yüzüncü Yıl University Gevaş Vocational School’s Students
Chicken meat is an important source of animal protein due to its cheap, high digestibility and biological value. The aim of this study is to determine the viewpoints and consumption habits on chicken meat of Van YYU Geva...
Effects of Nitric Oxide Application on Antioxidant Enzyme Activities of Pepper Plants under Drought Stress
The purpose of the study was to determine the relationship between the messenger molecule Nitric oxide (NO) and antioxidative enzyme (SOD: Superoxide Dismutase; CAT: Catalase; APX: Ascorbate Peroxidase) activities in som...
Yeast Functional Screen to Determine cDNAs of European Alkali Grass (Puccinellia distans) Conferring Selenium (Se) Toxicity
Selenium (Se), a metalloid, is essential for humans and animals, but its excess causes toxicity in all living organisms, including plants. Studies have shown that some wild plants such as European alkali grass (P. distan...
Determination of the Effect of Different Sowing Dates on Growth and Yield Parameters of Some Dry Bean (Phaseolus vulgaris L.) Varieties
The experiment was carried out at Dicle University, Faculty of Agriculture Department of Field Crops in 2018 spring and summer season. The aim of this study was to evaluate the effect of different sowing dates (March 10,...
Agricultural And Social Change In The Villages Of Kızıltepe District Of Mardin Province
The aim of this study is to reveal the agricultural and social change trends in families living in Akyazı, Sandıklı, Kuyucak, Demirkapı, Büyükdere and Alagöz villages of Kızıltepe district of Mardin province and engaged...