In vitro Propagation of Rhododendron griffithianum Wt.: An Endangered Rhododendron Species of Sikkim Himalaya
Journal Title: Journal of Applied Biology & Biotechnology - Year 2016, Vol 4, Issue 2
Abstract
A reproducible protocol for in vitro propagation of R. griffithianum has been established. Multiple shoot proliferation from shoot-tip explants was occurred in the presence of 2-isopentenyl adenine (2-iP) alone or in combination with indole 3-butyric acid (IBA) in Anderson's medium. The best treatment for shoot regeneration was Anderson's medium supplemented with 5 µM 2-iP and 1.0 µM IBA, which promoted shoot proliferation in more than 75% culture with an average of 11.00 shoots per explants after twelve weeks. These shoots were successfully rooted on Anderson's liquid medium supplemented with 1.5 µM IBA. The rooted plantlets were hardened in greenhouse with 84% survival rate.
Effect of GA3 treatments and sowing conditions on ex situ seed germination of Oroxylum indicum (L) Benth. Ex Kurz: A threatened high value medicinal plant
The objective of this study was to observe the effect of pre-sowing treatments of gibberellic acid (GA3 ) and different sowing conditions on ex situ seed germination of Oroxylum indicum. For this, after pre-sowing treatm...
Effect of gill removal and gutting on the quality of Tilapia (Oreochromis niloticus) under different storage condition
The study was conducted to investigate the effect of gill removal and gutting on the quality of Tilapia fish (Oreochromis niloticus) during different storage period using organoleptic, biochemical and microbiological asp...
RNA-Seq analysis reveals influence of sugar level and photoperiod on seasonality in oil palm (Elaeis guineensis Jacq.) sex-specific inflorescence emergence
We induced male inflorescence formation on 162 oil palm trees of 14 years old by complete defoliation. Inflorescence emergence data was analysed within a five-year period. Total RNA was extracted from un-emerged inflores...
Eco-friendly Industrial wastewater treatment: Potential of mesophilic bacterium, Pseudomonas putida (ATCC 49128) for hydrogen sulfide oxidation
The effectiveness and environmental friendliness of biological sulfide oxidation have endeared it to many researchers, mainly for its potential to offer the best alternative for the evacuation of various forms of sulfide...
Transcriptional expression of three putative pathogenesis-related proteins in leaves of rubber tree (Hevea brasiliensis) inoculated with Neofusicoccum ribis
Pathogenesis related-proteins (PR-proteins) and enzymes are important tools for understanding the molecular markers of plant response to external factors. In this study, semi-quantitative reverse transcription polymerase...