IN VITRO PROPAGATION OF VACCINIUM MYRTILLUS L.
Journal Title: Natural Resources and Sustainable Development - Year 2016, Vol 6, Issue 1
Abstract
The study aims to support a method of initiation in vitro cultures of seeds and minicuttings of bilberry (Vaccinium myrtillus) and to identify the effect of activated charcoal on Vaccinium myrtillus inoculums in subculture, to optimize rootedness or callus process, even in the absence of growth regulators. Initiation of in vitro cultures of bilberry (Vaccinium myrtillus) was successful, either from seeds or from minicuttings, on medium MB-MS - G with the addition of 2 mg/ l BA and 0.1 mg / l IBA. Activated charcoal 2% added to the in vitro bilberry culture medium led to stimulate only caulogenesis, but to decrease the survival rate of seedlings, by changing the pH of culture medium, which increases the value of 8, which favoured - in our laboratory conditions - the emergence of infections loving alkaline pH and loss of culture. In the step of multiplication of the callus, the activated charcoal led to lower its rate of necrosis, but also to changes in pH, as well. The initiation and proliferation of callus from bilberry was carried out under optimum conditions on culture medium with the addition of 3 mg /l 2.4 D, lacking activated charcoal, to yield a friable callus.
Authors and Affiliations
Anca Melania Nedelcu, Adriana Petruș-Vancea
Keywords
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