In vitro screening of immunomodulatory properties of synbiotics in chicken DT40 cell line
Journal Title: Animal Science Papers and Reports - Year 2016, Vol 34, Issue 1
Abstract
Synbiotics (prebiotic & probiotic) are used to stimulate the proper composition of the intestinal microflora in farm animals. One of the desired properties of synbiotics is connected with the immunostimulatory potential that allows for maturation of the immune system and regulation of its functions. This study aimed at selecting synbiotics with the most pronounced immunostimulatory properties, expressed by regulation of immune-related genes. For this purpose we used an in vitro assay based on the DT40 cell line stimulation with different synbiotics and downstream analysis of gene expression. We used a combination of three prebiotics: RFO, inulin, Bi2tos, and three probiotic bacteria strains of Lactococcus lactis. Stimulation was carried out at 37°C and in a 5% CO2 atmosphere for 9 hours. Downstream analysis of gene expression was performed at the mRNA level, using the RT-qPCR method. Each of the 20 analysed genes belonged to (1) cytokines/chemokines/regulatory molecules regulated by pre- and probiotics (i.e. IFN-γ, IFN-β, IL-4, IL-6, IL-8, IL-12p40, STAT4, CD3, CD80; iNOS), (2) the TLR2 signalling pathway (TLR2, MyD88) or (3) genes associated with the response of chickens to molecular patterns derived from gram-positive bacteria, i.e. lipoteichoic acid (MAPK8IP3, MAP2K4, MAP2K3, ITGB4, KLHL6, UNC13D and CARD11). The combination of the prebiotic inulin and probiotic Lactococcus lactis subsp. lactis SL2 provided the strongest regulation of the immune-related genes, which proves the immunostimulatory potential of this synbiotic.
Authors and Affiliations
Anna Sławińska, Maria Siwek, Marek Bednarczyk
Keywords
Related Articles
- EP ID EP81869
- DOI -
- Views 89
- Downloads 0
Effect of L-cysteine in extender on post-thaw quality of Sahiwal bull semen
The study was conducted to evaluate the effect of addition of L-cysteine to tris-citric acid (TCA) extender on the post-thaw quality of Sahiwal bull semen. For this purpose, two consecutive ejaculates were collected from...
Fatty acid composition of yolk of nine poultry species kept in their natural environment
Fatty acid (FA) composition of eggs from nine poultry species was determined chromatographically. Twenty six FAs were determined in the lipid composition of eggs. Monounsaturated fatty acids (MUFA) were found in higher a...
Performance, blood profile, carcass and meat traits and tissue morphology in growing rabbits fed mannanoligosaccharides and zinc-bacitracin continuously or intermittently
The study aimed at evaluation the effect of mannanoligosaccharides (MOS) and zinc-bacitracin (ZnB) administered continuously (+) or intermittently (+/-) upon growing performance, carcass and meat traits, blood profile an...
A relationship between somatic cell count, polymorphic form of β4-defensin and susceptibility of cow milk fat to lipolysis
The aim of this study was to evaluate the effects of somatic cell count and the polymorphic form of β4-defensin on the concentration of free fatty acids (FFA) and physico-chemical characteristics of cow’s milk. The study...
Adaptation of primer extension method for monitoring of scrapie-associated genotypes in sheep PRNP gene
The paper describes adaptation of primer extension method for genotyping scrapie-associated codons 136, 141 and 156 in sheep PRNP gene. As the method failed to genotype the polymorphism in codon 171 in some samples, this...