IN VITRO SELECTION OF Solanum Pimpinellifolium PLANT TOLERANT TO NaCl
Journal Title: Folia Pomeranae Universitatis Technologiae Stetinensis Agricultura Alimentaria Piscaria et Zootechnica - Year 2018, Vol 46, Issue 341
Abstract
Salinity is a major abiotic stress for plant worldwide which can reduce the average yields of most major crops such as tomato by more than 50%. The response of tomato to salinity is variable depending upon the line or cultivar. The aim of this study was carried out to determine the variation in salt tolerance for wild genotypes of tomato S. pimpinellifolium. To initiate callus tissue different combination of plant growth regulators were added to MS medium. The tolerant forms were selected at the callus stage and the stage of plants regenerated through somatic embryogenesis. Callus culture and shoot explants were exposed to different levels of salinity stress ranging from 0 (control) to 25, 50, 75, 100, 125 and 150 mM NaCl. The highest weight of dark green colour callus was observed on MS medium supplemented with 2.0 mg · dm–3 BAP and 2.0 mg · dm–3 IAA. It was shown that salt stress affected all growth parameters and addition to the MS medium 125 and 150 mM NaCl inhibited callus and somatic embryo initiation. The results obtained in this study suggested that S. pimpinellifolium somaclones isolated from callus selected on MS medium supplemented with 100 mM NaCl showed highest tolerance to salt stress.
Authors and Affiliations
Marcelina KRUPA-MAŁKIEWICZ, Danuta KULPA
Keywords
Related Articles
- EP ID EP412483
- DOI -
- Views 48
- Downloads 0
EPIGENETICS OF REPRODUCTION
Epigenetic research offers a significant opportunity to understand the role of environmental interactions on the genome and disease, while enabling modulation of these interactions in order to improve human health. Knowl...
CHANGES IN UDDER SKIN SURFACE TEMPERATURE OF DAIRY COWS CAUSED BY MACHINE MILKING
The aim of the present study was to indicate if machine milking significantly affects udder skin surface temperature (SST) to establish the most optimum time for SST measurement in mastitis detection. A total of 21 healt...
PRODUCTION PARAMETERS OF PHF COWS DEPENDING ON VARYING HOUSING CONDITIONS DURING LACTATION
The aim of the study was to determine the effect of varying housing conditions on production parameters of Polish Holstein-Friesian cows. The animals from the Experimental Station of the National Research Institute of An...
MONITORING AND PREVENTION OF LAMENESS IN A DAIRY CATTLE HERD
Hoof diseases in dairy cows maintained in the intensive production system occur with the same frequency as udder inflammation (mastitis), diseases of the reproductive system and metabolic disorders. Behaviour and motor a...
THE USE OF FLUORESCENCE SPECTROSCOPY TO ASSESS THE DEGREE OF OVERHEATING OF HONEY
The honey heating process used for its recrystallization (especially in a high temperatures and for a very long time) can result a chemical changes. The aim of this study was to use fluorescence spectroscopy to assess th...