In vitro shoot proliferation and rooting of Lallemantia iberica under Thidiazuron exertion
Journal Title: International Journal of Farming and Allied Sciences - Year 2016, Vol 5, Issue 3
Abstract
An in vitro culture of Lallemantia iberica was established for determination of optimum period for shoot production; for this purpose, the effect of thidiazuron (TDZ), as a cytokinin-like phenylurea compound, was investigated on in vitro shoot production and growth of L. iberica during every two weeks until six weeks of culture. TDZ in different concentrations (0.22-2 mg/l) was used as a supplement to the MS medium and nodal segments were employed as explants. Based on second and forth weeks investigation, low concentrations of TDZ could improve in vitro plant growth and 0.88 mg/l TDZ was found to be best productive for shoot (5.71±0.291), node (10.46±1.310) and leave (21.63±2.339) within four weeks, but in the third stage (6th weeks), TDZ had no effective function in comparison to control treatments. Based on the results obtained, increasing TDZ concentration decreased rooting percentage for Lallemantia iberica. The best rooting percentage (83.3±16.6%) was observed for free PGRs medium (control).
Authors and Affiliations
Nasrin Pourebad, Rouhollah Motafakkerazad, Morteza Kosari-Nasab, Ali Movafeghi, Nader Farsad Akhtar
Keywords
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