In Vitro Study Of Indirect Mechanism of Plant Growth Promotion
Journal Title: Indo American Journal of Pharmaceutical Research - Year 2011, Vol 1, Issue 5
Abstract
Nine isolates of soil bacteria were tested in vitro. Parameters assessed were HCN production, volatile compound production, production of non-volatile diffusible metabolite, protease enzyme production, antagonistic effect on phytopathogen. HCN production was tested qualitatively by picric acid and ammonium carbonate reagent and antagonistic activity is tested against stem rot causing pathogen sclerotium A. niger and A. flavus by dual culture technique. None of the culture showed HCN production and volatile compound production activity. Six cultures isolates NM/S3/NA, NM/S4/NA, NM/S6/NA, NM/S7/NA, NM/R2/RA NM/R3/RA showed protease production All cultures showed production of the non-volatile diffusible metabolite having inhibiting effect on phytopathogen A. niger whereas none of the bacterial isolates showed production of the non-volatile diffusible metabolite having inhibiting effect on phytopathogen A. flavus and Sclerotium. After three days of inoculation. The effect diluted after third day. Maximum inhibition showed by NM/S3/NA and minimum inhibition showed by NM/S1/CA and NM/S8/CA. Antagonistic activity is shown by culture NM/R3/RA against phytopathogen A. niger whilecultures NM/S4/NA, NM/S5/NA, NM/R2/RA showed minimum antagonistic activity as they are touching to the culture against phytopathogen A. flavus. None of the culture showed antagonistic activity against Sclerotium.
Authors and Affiliations
N A Ardhapurkar, A. V. Manwar
Keywords
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