In vitro study of the antimicrobial property of Green tea extract against standard (ATCC) bacterial strains and clinical isolates of Methicillin Resistant Staphylococcus aureus & Multidrug Resistant P
Journal Title: Indian Journal of Microbiology Research - Year 2016, Vol 3, Issue 3
Abstract
Objective: To evaluate the antimicrobial activity of Aqueous Green Tea extract against Standard ATCC Strains (Staphylococcus aureus ATCC 25923, Escherichia coli 25922, Pseudomonas aeruginosa 27853) & Clinical Isolates of Methicillin Resistant Staphylococcus aureus (MRSA) & Multidrug Resistant Pseudomonas aeruginosa (MDRPA). Methods: This Cross-sectional Analytical study was conducted in the Department of Microbiology of a Teaching Tertiary care hospital of Central India over a period of two months after obtaining Ethical clearance. Antimicrobial activity of green tea extract was determined by Agar well diffusion method. Results & Interpretation: The results of this study has shown that the aqueous extract of Green tea exhibits a potent antibacterial activity. Inhibition Zone diameter of green tea extract at lowest concentration (12.5 mg/ml) was 8mm, 10mm & 10mm respectively for ATCC strains of E.coli, Pseudomonas aeruginosa & Staphylococcus aureus. However at highest concentration (200mg/ml) it was 20mm, 22mm & 26mm respectively. Amongst six MRSA isolates tested aqueous green tea extract exhibited significant antibacterial activity against five isolates. Amongst six MDRPA isolates tested even at lowest concentration of 12.5 mg/ml aqueous green tea extract showed appreciable antimicrobial activity against four isolates. Conclusion: Aqueous extract of Green tea (Camellia sinensis) prepared by cold extraction method do have a significant antibacterial activity not only against standard ATCC bacterial strains but also against highly resistant clinical isolates of MRSA & MDRPA.
Authors and Affiliations
Nayan Dubey, Abhishek Mehta
Keywords
Related Articles
- EP ID EP241143
- DOI -
- Views 113
- Downloads 0
Is commercial control necessary as a third party internal control for HIV – antibody ELISA testing? A comparative study in NABL accredited laboratory
Aims & Objectives: To assess and compare the feasibility, availability, affordability, stability of In-house and commercial third party internal control of HIV antibody ELISA test. Methods: A comparative study was conduc...
HIV co-infection among Pulmonary Tuberculosis infected persons in and around District of Kancheepuram, Tamil Nadu, India
Despite the implementation of a widely adopted strategy to control tuberculosis (TB), the disease remains a major public health problem particularly in developing country, like India. The objective of the study was to fi...
Evaluation of multiple antibiotic resistance (MAR) index and Doxycycline susceptibility of Acinetobacter species among inpatients
Background: Acinetobacter contributes to increased morbidity and mortality with its strong propensity to colonize and disseminate among humans and environmental sources coupled with its ability to develop resistance to a...
“Enterococci speciation and antibiogram: an assessment of data at a tertiary care hospital in Karnataka”
Background: Enterococci were known to be less virulent in healthy individuals, but recently have become important opportunistic pathogens, especially in hospitalized patients because of their ability to colonize the gast...
Capability of Aspergillus niger to bioconcentrate cesium-137 and cobalt- 60 from medium and low level radioactive waste solution simulates
Introduction: The bioremediation, as a treatment process for low- and medium radioactive liquid wastes generated from various applications of nuclear technology in our daily life, represents a biotechnological innovation...