IN-VITRO STUDY ON POTENTIAL EFFICACY OF ACRIFLAVINE IN THE PREVENTION OF CONJUGATIONAL TRANSFER OF DRUG RESISTANCE IN BACTERIA
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2017, Vol 6, Issue 78
Abstract
BACKGROUND Acquisition of new resistance in bacteria is brought into effect by a battery of gene transfer systems, among which conjugation accounts for bulk of horizontal gene transfer. Acriflavine is a DNA-intercalating agent with plasmid curing properties. Since it selectively inhibits plasmid replication, it might have a role in interfering with conjugation. The aim of this study was to observe for any in-vitro inhibitory effect of Acriflavine on horizontal transfer of resistance factors. MATERIALS AND METHODS Overnight BHI broth cultures of donor Escherichia coli and recipient Escherichia coli strains were taken and experiment done simultaneously in following steps. Mating between donor and recipient strain was done by broth mating protocol, and resultant transconjugant colony count (TCC) was calculated and compared between Acriflavine treated and untreated mating mixtures. The bacteriostatic effects were measured and viable colony count (VCC) was performed separately on Acriflavine treated and untreated broth cultures of both donor and recipient. Standard plasmid curing protocol using Acriflavine was performed on donor strain and curing efficacy were calculated. Five repetitions were done for each conjugating pair, and median values were used for further analysis by descriptive statistics. RESULTS The % decrease of TCC was greater than % decrease attributed to combined effect of bacteriostasis/curing. This difference hints towards the presence of some inhibitory effects other than bacteriostasis and curing. CONCLUSION Acriflavine being a strong DNA-intercalating agent, perhaps has some negative effect on conjugation process which needs to be further investigated. If proven, Acriflavine & other similar compounds will open up the possibility of being used as adjuvant therapy along with antimicrobial agents to slow down the rate of development of new resistance and increased longevity of antibiotic efficacy
Authors and Affiliations
Tamas Das, Bipasa Chakraborty, Raja Ray
Keywords
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- EP ID EP268045
- DOI 10.14260/jemds/2017/1204
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