In vitro transfection potential of fluorinated G5 PAMAM dendrimers for miRNA delivery to MRC-5 cells
Journal Title: The European Research Journal - Year 2018, Vol 4, Issue 2
Abstract
Objectives. MicroRNAs (miRNAs) are involved in the regulation of most biological processes and also contribute to many types of disease. Fibroblast cells, such as MRC-5, are often used in biological researches utilizing cell transfection methods due to their difficult to transfect nature. Cells can be genetically engineered by using viral and non-viral methods. Poly(amidoamine) (PAMAM) dendrimers are very promising alternative as a delivery vehicle due to their well-defined characteristics. In this study, in vitro transfection potential of cystamine core generation five (G5) PAMAM dendrimers fluorinated with 2,3,4,5,6-pentafluorobenzoic acid (PFB) and pentafluoropropionic acid (PFP) for miRNA delivery to MRC-5 cells was examined. Methods. Spectroscopic techniques were used in the characterization of the prepared dendrimers. miRNA binding and condensation capability of dendrimers was examined by gel retardation assay. Characterization of dendriplexes was made by zeta potential, particle size measurements and transmission electron microscopy. Transfection efficiencies of the dendriplexes were determined by flow cytometry and intracytoplasmic distribution of the dendriplexes was shown by laser scanning confocal microscopy. Also, quantitative structure-activity relationship and molecular docking calculations were used to be able to discuss transfection efficiencies of the dendriplexes into the cell. Results. While high level of viability on MRC-5 cells was observed for dendriplexes prepared with PFB and PFP, transfection efficiency with PFP was higher than PFB. Transfection efficiency difference between these two compounds was attributed to their molecular structures. Conclusions. Obtained results hold promise for the usage of these compounds as a transfection reagent at MRC-5 cells. Further studies are needed to support these findings.
Authors and Affiliations
Ali Öztuna, Hasan Nazır
Keywords
Related Articles
- EP ID EP278948
- DOI 10.18621/eurj.346612
- Views 78
- Downloads 0
Mitral valve myxoma associated with intracranial tumor: a case report
Primary tumors of the heart are rare, and among them, cardiac myxoma is the most prevalent primary cardiac neoplasm in adult patients. Astrocytoma is the most common glioma and can occur in most parts of the brain and oc...
The role of inflammation markers in predicting the prognosis of Bell's palsy
Objectives: This study aimed to investigate the relationship between the neutrophil-to-lymphocyte ratio (NLR) and the platelet-to-lymphocyte ratio (PLR) and Bell’s palsy in addition to the usability of these markers to p...
An unusual cause of hemorrhagic shock: gastric Dieulafoy’s lesion
Dieulafoy's lesion (DL) related massive gastrointestinal bleeding is a rare and mortal health condition. DL is a superficial vascular lesion of the gastric mucose that is mostly located in the proximal part of the stomac...
Gender dysphoria in an adolescent diagnosed with Klinefelter syndrome over a follow-up period
Although genetic factors have been thought to be main cause for gender dysphoria, its etiology is still not clearly understood. Klinefelter syndrome is the most seen sex chromosomal disorder. In the literature, there are...
A challenging palliation via novel therapeutic approach for unusual presenting with gingival metastasis from renal cell carcinoma relapsed eight years after nephrectomy
Renal cell carcinoma (RCC) is the third most frequently seen primary source of metastatic lesions of the oral cavity. Still oral cavity metastasis is a rarely seen clinical manifestation during the course of RCC. In a pa...