Abstract

Objective: To study the in vitro interactions of ibuprofen with enalapril. Methods: In this study, we investigated synergistic effect of angiotensin-converting enzyme inhibitor (enalapril) with commonly used nonsteroidal anti-inflammatory drug, ibuprofen in carrageenan induced inflammation rats. Anti-inflammatory response was observed for ibuprofen when given simultaneously with enalapril by comparing decrease in paw size. Results: The results were expressed in percentage reduction in paw size for every hour and were calculated for edema rate and percentage reduction in inflammation. In vitro interaction studies were carried out using high performance liquid chromatography and both the drugs were analyzed by assaying both drugs alone and in combination. The combination of enalapril and ibuprofen showed increased action of ibuprofen as shown by the increase of percentage reduction and decrease in edema rate. It was also proved from analytical ultracentrifugation of the chromatogram of combining drugs. Conclusions: This learning has certain restriction as a number of experienced animals were small and the period of inflammatory response was checked for ibuprofen alone and simultaneous with enalapril for 5 h. Further studies may be required to establish the strong relationship.

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