In Vivo Anti-Trypanosomal Potential Of Methanol Root Extract Of Terminalia Macroptera (Guill. And Perr.) In Trypanosoma Brucei Brucei Infected Wistar Rat
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 5
Abstract
Trypanosoma brucei brucei is a haemo-protozoan parasite transmitted by tsetse fly, which causes African animal trypanosomiasis (AAT), a parasitic disease that causes economic loss in cattle, sheep and goats. Existing drugs for the management of AAT have been reported to be expensive, toxic and rapidly becoming ineffective due to animals becoming resistant to the drugs. Hence the need for drugs of plant origin that are natural, easily accessible, and cheaper, with minimal side effects when compared to conventional antitrypanocidal drugs. Terminalia macroptera (T. macroptera) is an ethnomedicinal plant used as antimicrobial and antimalarial therapies in some developing countries.The study aimed at evaluating the anti-trypanosomal potential of the methanol root extract of T. macroptera in Wistar rats. A prior Gas Chromatography Mass Spectrometry (GCMS) was carried out on the root extract. Twenty-five female Wistar rats were used for the study. These were divided into five groups (I: Therapeutically treated; II: Positive control; III: Prophylactically treated; IV: Extract control and V: Healthy control) of five animals each. Experimental groups I and III received 64 mg/kg of extract after and before a parasite dose of 106 respectively, via intraperitoneal route. Results obtained showed that Groups I and III had insignificant parasitaemia peaks with 40% and 20% survival respectively, at day 11 post infection (Pi), as compared to Group II which had a significant parasitaemia peak with 0% survival at day 6 Pi. The extract and healthy controls had 100% survival at day 14. Furthermore, Haematoxylin and Eosin stained spleen, liver and kidney tissues revealed distortion in the Group II as compared to Groups I and III. No significant (P > 0.05) change in body weight was observed. Methanol root extract of T. macroptera regulated parasite multiplication, ameliorated histopathological and hematological changes (P < 0.05) and extended animal survival in experimental trypanosomiasis.
Authors and Affiliations
Balogun, J. B. *, . , Abubakar , Z1, Ibrahim ,T. B, Sadiq, I. S3, Dogara, M. M1, , Idoko, S. I6, Owolodun , H. O4, Orendu, M. A5
Keywords
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- EP ID EP391250
- DOI 10.9790/3008-1205061217
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