In vivo Evaluation of Microorganisms Isolated from Peels of Selected Carbohydrate Rich Tubers
Journal Title: Microbiology Research Journal International - Year 2016, Vol 14, Issue 5
Abstract
Aims: To detect the effect of microbes isolated from peels of Carbohydrate rich tubers on Albino rats. Study Design: The study was a comparative and descriptive study. Cassava and cocoyam peels were bought from Obanla market, Federal University of Technology, Akure. Those samples were put into a sterile polythene bag and transported to microbiology laboratory for microbiological analysis. Place and Duration of Study: Microbiology Laboratory, Elizade University, Department of Animal Production and Health Research Laboratory, Federal University of Technology, Akure, Ondo State. The study was performed between September, 2015 and March, 2016 Methodology: Two gram of the samples was grounded in a mortar with pestle. The serial dilution method was aseptically used to reduce the microbial load present in the collected samples. Nutrient agar and potato dextrose agar were used to isolate the bacteria and fungi respectively from the samples under aseptic condition. Biochemical test and sugar fermentation were carried out on the bacterial isolates after pure culture was obtained. Results: The isolated bacteria were Streptococcus faecalis, Corynebacterium fascians, Micrococcus luteus, Alcaligenes faecalis and Aeromonas hydrophila. Fungal isolates were identified using lactophenol cotton blue. Fungi isolated includes: Articulospora inflata, Aspergillus fumigatus, Aspergillus niger, Geotrichum albidum, Torula herbarum and Halosporangium panum. All the bacteria isolates were used to infect Albino rat. The uninfected rats were used as control. The histopathological and haematological analysis carried out on liver and intestine of the albino rats showed that the microorganisms caused a pathological change that ranges from necrosis of the liver hepatocytes, hepatocellular drainage and hemorrhage to vacuolation and inflammatory cell infiltrations of the intestinal wall. Conclusion: This study has shown that cassava and cocoyam peels can act as vehicles for disseminations of pathogenic microorganisms and should therefore be properly disposed.
Authors and Affiliations
A. O. Momoh, O. S. Fadare
Keywords
Related Articles
- EP ID EP350909
- DOI 10.9734/BMRJ/2016/26177
- Views 74
- Downloads 0
Hepatitis C Virus Infection among Chronic Renal Failure Patients Pre – Dialysis Therapy in Hodiedah City, Yemen
Background: Hepatitis C virus (HCV) infection is a significant problem in hemodialysis patients, which poses difficulty in the management of the patients in the renal dialysis unit. Objective: This study aimed to detect...
Preparation of Fowl Typhoid Vaccine from Field Isolates and Determination of Efficacy
The experiment was conducted to isolate and identify Salmonella gallinarum from field cases to prepare formalin killed vaccine and to determine the efficacy of experimentally prepared fowl typhoid vaccine. A total of 48...
Role of Chronic Viral Hepatitis B and C as Risk Factors for Celiac Disease
Aims: Celiac disease (CD) is an autoimmune disease and hepatitis B virus (HBV) is suspected to trigger immunologic gluten intolerance in susceptible individuals. The aim of this study is to assess the role of chronic HBV...
Response Surface Methodology for the Optimization of Proteases Production by a Novel Egyptian Isolate Bacillus amyloliquefaciens 35s
Aims: The present work aimed to optimize proteases production by Bacillus amyloliquefaciens 35s using the response surface methodology (RSM). Study Design: Variables affecting proteases production were screened using a P...
An in-vitro Analysis of the Antioxidant and Antimicrobial Properties of the Methanol Extract of Hypoxis hemerocallideacorm (MEHHC) from Botswana
Aims: To evaluate the antimicrobial and antioxidant activity of the methanol extract of Hypoxis hemerocallidea corm (MEHHC) with different assays. Study Design: In vitro evaluation of antibacterial and antioxidant activi...