Increased ROS Generation: Implication in Antibacterial Activity of Evolvulus nummularius against Multidrug Resistant Gram Negative Bacterial Strains
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2017, Vol 6, Issue 1
Abstract
Evolvulus nummularius, is ethnomedicinally used as a medicine for hysteria, to cure burns, cuts, wounds and scropion stings. This plant has antihelminthic activity, wound healing activity, poor sedative and anticonvulsant properties. In this study, the antibacterial activity of crude methanol extract of E. nummularius was studied against three gram negative multidrug resistant strains E. aerogenes, P. aeruginosa and K. pneumoniae. This plant extract showed both bacteriostatic and bactericidal activity against all these bacterial strains. The extract was most active against E. aerogenes. In growth analysis study, it was obtained that, after treatment with IC50 dose for each bacterial strains, the lag phase become extended compared to untreated bacterial cells. To understand the mechanism of action of Evolvulus nummularius the reactive oxygen species (ROS) was estimated and result showed that, reactive oxygen species were increased (~ 35-60%) in presence of IC50 dose of Evolvulus nummularius.
Authors and Affiliations
Susmita Saha, Anindya Das, Angkita Debnath, Shipa Begam, Subrata Sen, Swapan Majumdar, Samir Kumar Sil
Keywords
Related Articles
- EP ID EP566773
- DOI 10.20546/ijcmas.2017.601.013
- Views 46
- Downloads 0
Molecular Analysis of Micropropagated Banana Variants (Musa spp. ‘AAA’) Using RAPD Markers
In this study, RAPD fingerprinting has been carried out for variants selected from orchards growing with micropropagated Robusta banana (Musa spp. ‘AAA’) plants. After isolation of quality DNA and preliminary screening o...
Anti-Insect Activities of Plant Extracts on the Diamondback Moth, Plutella xylostella (L.)
The diamondback moth is known globally by many as the most destructive and economically important insect pest of cruciferous crops. It is also known to have developed resistance to numerous synthetic insecticides includi...
Efficacy of Organically Managed Cropping System in Improvement of Soil Health in Ne Hill Region
Field experiments were conducted during the kharif seasons of 2015 and 2016 to evaluate the efficacy of the organically managed cropping system in improvement of soil health in NE hill region. Four combinations of two cr...
Entrepreneurial Behaviour of Dairy Farmers: A Study in Marathwada Region of Maharashtra, India
A study was conducted in Aurangabad and Jalna district of Marathwada region of Maharashtra to know the level of entrepreneurial behaviour of dairy farmers. A sample of 120 respondents was selected for present study. The...
Identification and Antimicrobial Susceptibility testing of Non Fermenting Gram Negative Bacteria by Vitek 2 in a Teaching Hospital in Mahbubnagar
Non fermenting gram negative bacilli (NFGNB) are aerobic, non-spore forming bacteria. Although previously considered as contaminants, their pathogenic potential was proved by their frequent isolation from clinical sample...