INCREASED SPERM DNA FRAGMENTATION NEGATIVELY AFFECTS EMBRYO QUALITY IN A COHORT OF EGYPTIAN PATIENTS.
Journal Title: International Journal of Advanced Research (IJAR) - Year 2019, Vol 7, Issue 1
Abstract
Sperms of infertile men mostly contain more DNA damage than fertile males. Sperm DNA damage might have a negative effect on the fertility potential of such patients. This study measuredthe percentage of spermatozoa with nuclear DNA fragmentation in semen samples used for intracytoplasmic sperm injection (ICSI), before and after sperm processing and investigated the association between sperm DNA fragmentation index (DFI) and ICSI outcomes (embryo quality and clinical pregnancy rate).Sperm DFI was measured, by TUNEL, in fresh semen samples obtained from thirty male partners of infertile couples, prepared for ICSI.The mean sperm DFI before and after processing was 4.5% and 8.83% respectively. Sperm DFI increased significantly after processing (P< 0.001) regardless of the type of processing used (Z= 0.155, p= 0.877). The correlation between sperm DFI, before and after semen processing, and clinical pregnancy was statistically insignificant (Z = 0.4, p = 0.689 and Z= 0.48, p = 0.631 respectively). DFI of sperm after semen processing showed a significant negative correlation with embryo grading at day 3 (r = - 0.232, p = 0.002). In conclusion, sperm DFI increases significantly after processing and correlates negatively with embryo quality.
Authors and Affiliations
Gaafar Sh, El-Geziry D, Younan D, El-Dakhakhny M.
Keywords
Related Articles
- EP ID EP458654
- DOI 10.21474/IJAR01/8323
- Views 70
- Downloads 0
ILYAS EFENDIYEVS DRAMATURGIC LANGUAGE ASPECTS AND ITS FORMATION ISSUES.
The main aim of the research is to investigate the dramaturgic language aspects and their formation issues in creation of Ilyas Efendiyev – one of the outstanding classicists of Azerbaijan literature of XX century. Ilyas...
ROLE OF ENDOSCOPY IN EVALUATING UPPER GASTROINTESTINAL TRACT LEISONS AT A TERTIARY CARE HOSPITAL.
BackgroundandObjectives: Endoscopy as a diagnostic and therapeutic tool has grown in recent years. Upper gastrointestinal (GI) endoscopy is one of the most fascinating branch which serves not only as a means of resolving...
ACUTE COCAINE MYOCARDITIS IN A YOUNG HEALTHY PATIENT.
Cardiac arrhythmia and acute myocardial ischemia are the most common causes of cocaine-induced sudden cardiac death. However, cocaine induced myocarditis is uncommon. We report a case of a 22-year-old male with no signif...
PROSTHODONTIC MANAGEMENT OF NEUROLOGICALLY COMPROMISED PATIENTS.
International Journal of Advanced Research (IJAR)
THE EFFECTS OF THE OLIVE OIL COMPONENTS ON REDUCING THE CARDIOVASCULAR COMPLICATIONS.
Background: Cardiovascular diseases are responsible for more than 7 million deaths worldwide. Cardiovascular risk factors such as hypertension, diabetes and smoking, contribute to the onset of the inflammatory process in...