Indicators of Metabolic Endotoxemia in Ovarietomated Rats with Metabolic Syndrome during Pharmacological Correction
Journal Title: Український журнал медицини, біології та спорту - Year 2017, Vol 7, Issue 5
Abstract
Nowadays the problem of metabolic endotoxemia is widely discussed in scientific literature. Connection between hormonal and particularly estrogen status and metabolic endotoxemia had been scientifically proved. However, there is no sufficient justification of the immunotropic compounds and exogenous estrogen influence on the state of Endotoxemia during postovariaectomia metabolic syndrome. In the laboratory of hormone-like compounds synthesis of the State Institution “V. Danilevsky Institute for Endocrine Pathology Problems of National Academy of Medical Science of Ukraine” (Kharkov, Ukraine) the derivative of levamisol-308 (PL-308) which corresponds to the class 5 of toxicity (almost non-toxic compounds) according to the acute toxicity data had been synthetically produced. The purpose of the research was to determine the state of metabolic endotoxemia in ovariectomized rats during metabolic syndrome (MS) for liposaccharide (LPS) antibodies and introducing compounds with immunomodulatory properties of levamisole and its derivative PL-308 and exogenous 17β-estradiol. Materials and Methods. The tests were conducted on the 3-month white female rats of the Vistar line. We reproduced the hypoestrogenia by the way of two-side overiaectomia with the light etherization. We preceded the induction of MS within 2 weeks after the operative intervention. The high-carbohydrate diet (HCD) was provided by the free access to the 30% sweet water as a potable water during 5 and 8 weeks. Levamisole and its derivative PL-308 were provided for 5 weeks by the means of a zonde. 17β-estradiol was provided intramuscularly once daily for two days (10 μg / kg body weight) for 8 weeks. The state of metabolic endotoxemia was assessed by the level of antibodies to the endotoxin, which were determined by ELISA method. Results. Hypoestrogenia is an independent reason of antibody level increase of LPS. The combination of estrogen deficit with the MS didn`t influence the endotoxemia level. The compounds with immunomodulation capacities as levamisole and its derivative PL-308 have decreased the antibody level to LPS with the ovariaectomia rats with MS to the level of intact animals, when 17β-estradiol has decreased but not normalized the endotoxin level with the similar group of rats. Conclusions. The normalization of LPS level which was observed only during levamisole usage and its derivative PL-308, testifies that the immune correction is an important step in women rehabilitation with the metabolic misbalance and is crucial to the metabolic endotoxemia decrease along with the protective treatment of the immune deficit conditions.
Authors and Affiliations
V. Kozar, R. Yeriomenko, O. Dolzhykova, N. Gorbenko, M. Kudrya, F. Yaremenko
Keywords
Related Articles
- EP ID EP266201
- DOI -
- Views 132
- Downloads 0
Structural-Functional Changes in the Colon’s Wall under Conditions of Sodium Glutamate Usage
The purpose of the study is to examine morphological changes of mucosa of rats’ colon under the influence of monosodium glutamate (MSG). Materials and methods. Studies were conducted on white nonlinear male rats weighing...
Zinc Citrate Influence on Antioxidant Defence in Rats’ Liver and Pancreatic Gland during Experimentally Diabetes
The purpose of the article is to present data towards the zinc citrate action of on the activity of antioxidant system enzymes and the content of reduced glutathione and products of peroxide oxidation of lipids in liver...
УСТРАНЕНИЕ СТУПЕНЧАТОГО УСТУПА В ИЗОГНУТОМ КОРНЕВОМ КАНАЛЕ ПРИ ПОВТОРНОМ ЭНДОДОНТИЧЕСКОМ ЛЕЧЕНИИ ЗУБОВ, РАНЕЕ ОБТУРИРОВАННЫХ РЕЗОРЦИН-ФОРМАЛИНОВЫМ МЕТОДОМ
Создание ступенчатого уступа при эндодонтическом лечении является ятрогенным осложнением. Под уступом понимают искусственно сформированное отклонения от естественной траектории корневого канала без сообщения с периодонто...
Psychological Characteristics of Perception of Athletes Specializing in Water Sports
The article suggests the analysis of the main forms of information perception (visual, auditory, kinesthetic) in athletes’ specializing in water sports. It also identifies the relationship between the leading psychologic...
ФЕТАЛЬНА МІКРОСКОПІЧНА АНАТОМІЯ СІМ’ЯНИХ ПУХИРЦІВ
У статті за допомогою мікроскопічного методу дослідження вивчені особливості структурних змін шарів стінки сім’яних пухирців упродовж плодового періоду онтогенезу людини. Епітелій слизової оболонки сім’яних пухирців пред...