INDUCED MUTAGENESIS IN ABELMOSCHUS MOSCHATUS (L.) MEDIK
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 5
Abstract
Dry seeds (cultivar I – tall, branched, moisture content 3.5%; cultivar II – dwarf, unbranched, moisture content 1.5%) of Abelmoschus moschatus (L.) Medik (Family: Malvaceae; Common name: Ambrette; English name: Musk mallow) were treated (0.25, 0.50, and 1.00% for 3h and 6h durations) with chemical mutagens ethyl methane sulphonate (EMS) and hydroxylamine (NH2OH) to raise desirable ‘plant type’ mutants. Mutagenic sensitivity (from M1 biological damages like lethality, injury and sterility; LD50 was ascertained), M2 mutation frequency and types (cv.I: pigmented stem, lax branching, long petiole, large flower and early flowering, 1089 plants scored; cv.II: funnel, thick stem and late flowering, 1945 plants screened), mutagenic effectiveness (EMS more effective than NH2OH) and efficiency (variable response but threshold dose seems to be efficient) and meiotic chromosome behaviour of the plant types were assessed. Macromutants evolved were viable and may be important genetic resources in the plant species.
Authors and Affiliations
Kumari Dubey , Animesh Datta
Keywords
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