Induced mutation and in vitro techniques as a method to induce salt tolerance in Basmati rice (Oryza sativa L.)<br />
Journal Title: International Journal of Environmental Science and Technology - Year 2005, Vol 2, Issue 2
Abstract
Embryogenic callus of indica rice (Oryza sativa L.) cv. Basmati 370 induced on MS medium containing 9.05 M 2, 4-D was irradiated at 50 Gy of gamma rays of 60Co for creating genetic variability against salinity. Irradiated and non-irradiated calluses were screened in vitro through three consecutive proliferation phases at 4.0, 6.0, 8.0 and 10.0 d/Sm electrical conductivity of NaCl. Growth value and number of adapted mutagenized callus was more than that of non-mutagenized callus. Salinity levels beyond 6 d/Sm and 8 d/Sm were lethal to growth and adaptation of non-irradiated and irradiated callus respectively. NaCl adapted irradiated callus showed 2.0%-4.75% regeneration frequency on MS regeneration medium containing 5.37 M NAA and 9.29 M Kinetin. Non-mutagenized salt adapted callus did not show any regeneration. From gamma ray mutagenized cultures, 2 putative lines (M2 generation) with moderate salt tolerance were obtained at seedling stage. The results suggest that in vitro technique in connection with gamma rays may be used as a versatile approach to improve the level of salt tolerance in Basmati rice for saline environment.
Authors and Affiliations
M. Y. Saleem, Z. Mukhtar, A. A. Cheema, B. M. Atta
Keywords
Related Articles
- EP ID EP154744
- DOI -
- Views 114
- Downloads 0
Control of disinfection by products formation potential by enhanced coagulation
Jar-test experiments were conducted to study enhanced coagulation effectiveness in removal of disinfection by products (DBPs) from Zayandehrud River at Isfahan Province-the center part of Iran- in 2004. In this study, th...
Impact assessment due to rural electrification in hill tract of Bangladesh for sustainable development
Environmental impact assessment (EIA) of any project is essential for understanding the sustainability of the project. For sustainable development of hill tracts, electricity is inseparable. Like other parts of Banglades...
The comparative study of trihalomethanes in drinking water
The purpose of this study was to assess exposure of four trihalomethanes (chloroform, bromodichloromethane, dibromochloromethane and bromoform) in drinking waters of Okinawa Island and Samoa. Trihalomethanes compounds we...
Alteration of macroinvertebrate community in tropical aquatic systems in relation to sediment redox potential and overlaying water quality
Limnological studies in two tropical Indian aquatic habitats showed that macroinvertebrate communities have greater diversity than other biotic communities present there. Sediment redox potential is found to be an import...
Assessment of cement’s dust impact on the soil using principal component analysis and GIS<br />
To evaluate the impact of the cement’s dust emitted by the eastern Moroccan cement factory (Oujda Holcim) and deposited on the soil of the Ain Lahjar commune, 58 samples of soil were collected around the factory on a rad...