Induction of callogenesis and organogenesis of different melon genotypes
Journal Title: Journal of BioScience and Biotechnology - Year 2017, Vol 6, Issue 2
Abstract
The effect of different concentration of BA and Kinetin on regeneration of cotyledons, hypocotyls and leaves of four melon genotypes was studied. It was established that cotyledons and leaf segments were more effective as explants for bud induction and subsequent plant regeneration than hypocotyls. In the studied melon genotypes, the better regeneration was obtained in medium variants supplemented with growth regulator BA. The best response was observed in true leaves (70.0%) and cotyledons (53.3%) from line 5-1-2 in medium variant containing 1.0 mgL-1 BA. On medium variants containing Kinetin the regeneration efficiency was lower. Data from threeway analysis of variance confirmed that regeneration ability is strongly influenced by explant type and interaction of both factors - genotype and explant type. The highest number of plantlets/explant was registered in leaf segments from line K052 (0.9), followed by cotyledons and true leaves from line 5-1-2 (0.7) in medium variant contained 1.0 mgL-1 BA.
Authors and Affiliations
Zhana Ivanova, Stanislava Grozeva, Nikolay Velkov
Keywords
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