Induction of callus from leaf and petiole of the plant Coleus vettiveroids K .C. Jacob
Journal Title: Scholars Academic Journal of Biosciences - Year 2013, Vol 1, Issue 6
Abstract
Coleus vettivereoids K .C. Jacob belonging to the family Labiatae is a widely accepted plant known for its traditional uses such as antipyretic, antibacterial, anti-inflammatory etc. The present study aimed to evolve a protocol for rapid multiplication using culture technique. Tissue explants like leaf lamina and petiole were cultured on MS media supplemented with different concentrations of 2.4-D (0.5-8 mg/l), IAA (0.5-8mg/l) and their callusing were studied. A proper method developed for surface sterilization of explants because the major problem in the development of a callus in the case of Coleus species is the contamination resulting from its hairy nature. 0.1% w/v HgCl2 for 1 min application followed by ethyl alcohol (70%) for 30 seconds was found to be most effective. The MS basal + 2, 4 D (1.5 mg/l) and MS Basal +IAA (2.00 mg/l) showed induction of callus from leaf explants and MS basal + 2,4D (1.5mg/l) showed induction of callus form petiole. Keywords: Callus culture ,Coleus vettiveroids, Hrivera, Labiatae, ,leaf, petiole
Authors and Affiliations
Beesha S Kamal, Padmaja V, Biju Dharmapalan
Keywords
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