Influence of composition on the biological activity of pectic polysaccharides from leek
Journal Title: Journal of BioScience and Biotechnology - Year 2013, Vol 2, Issue 1
Abstract
Water-soluble and acid-soluble pectic polysaccharides with significant immunostimulating activity were isolated from alcohol-insoluble solids (AIS) of leek. Pectic polysaccharides characterized with high anhydrouronic acid content and common pectic sugars – L-arabinose, D-galactose, D-glucose, and L-rhamnose. It was accomplished that D-galactose content was the highest, which allowed the assumption that pectin sugar side chains were from galactan type. Enzyme modification of acid-extracted pectin was realized. Different pectin-degrading enzymes were used. The first modified pectic fraction, obtained after endopolygalacturonase action and alcohol precipitation contained rhamnogalacturonan I and had high D-galactose content. The second fraction obtained from the first, after β-galactosidase treatment characterized with low D-galactose content. Probably this was result from β-galactosidase hydrolysis of pectin side chains. It was proved by the action of β-galactosidase that galactan side chains were mainly composed of β-(1→4)-linked D-galactose. It was accomplished that rhamnogalacturonan I had higher immunostimulating activity than the initial pectic polysaccharide, which confirmed the statement that pectin hairy regions were responsible for the biological activity. The second fraction had lower D-galactose content and lower activity than the first. Probably galactan side chains were responsible for the immunostimulating activity.
Authors and Affiliations
Manol Ognyanov, Mariana Nikolova, Irina Yanakieva, Veselin Kussovski, Maria Kratchanova
Keywords
Related Articles
- EP ID EP103990
- DOI -
- Views 63
- Downloads 0
Effects of the insecticide "Actara 25 WG" on the glyconeogenesis in the liver of common carp (Cyprinus carpio L.)
The main goal of the present work is to study the effects of the new neonicotinoid insecticide „Actara 25 WG" on the intensity of expression of glycogen in the liver of common carp (Cyprinus carpio L.) by using PAS-react...
3D structure prediction of lignolytic enzymes lignin peroxidase and manganese peroxidase based on homology modelling
Lignolytic enzymes have great biotechnological value in biopulping, biobleaching, and bioremediation. Manganese peroxidase (EC 1:11:1:13) and lignin peroxidase (EC 1:11:1:14) are extracellular and hem-containing peroxida...
Approaches for prediction of the implantation potential of human embryos
Optimization of assisted reproductive technologies (ART) has become the main goal of contemporary reproductive medicine. The main aspiration of scientists working in the field is to use less intervention to achieve more,...
Biosorption of Cu (II) onto chemically modified waste mycelium of Aspergillus awamori: Equilibrium, kinetics and modeling studies
The biosorption potential of chemically modified waste mycelium of industrial xylanase-producing strain Aspergillus awamori for Cu (II) removal from aqueous solutions was evaluated. The influence of pH, contact time and...
GC-MS based metabolite profiling of five Bulgarian Fumaria species
The aim of this study was profiling of primary metabolites of five Bulgarian Fumaria species (F. officinalis L., F. thuretii Boiss., F. kralikii Jord., F. rostellata Knaf. and F. schrammii Velen.) by GC-MS analyses...