Influence of different levels of heparin and antithrombin Ⅲ on human coagulation factor Ⅸ activity
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 6
Abstract
Objective To study the effect of different concentrations of heparin, ATⅢ or a mixture of heparin and antithrombin Ⅲ (ATⅢ) (1∶1)on the activity of human coagulation factor Ⅸ (FⅨ). Methods The heparin or heparin/ATⅢ with different concentrations were added into human coagulation Ⅸ products or human prothrombin complex (PCC) to prepare heparin or heparin/ATⅢ samples, containing 0, 0.1, 0.3, 0.5, 0.8, 1, 2 and 4 IU per unit. ATⅢ with different concentrations were added into FⅨ or PCC to prepare ATⅢ samples containing ATⅢ 0, 0.1, 0.5 and 1 IU per unit. The FⅨ activity of the samples prepared was tested by one-stage coagulation method. Then corresponding amount of protamine sulfate were added to neutralize heparin or heparin/ATⅢ to detect the FⅨ activity again. Their influence of heparin, ATⅢ and heparin/ATⅢ with different concentrations on the activity of FⅨ were analyzed. Results When the content of heparin or heparin/ATⅢ was 0, 0.1, 0.3 and 0.5 IU per unit of FⅨ, the detection results of FⅨ titer in samples were consistent. When the content of heparin or heparin/ATⅢ per unit of FⅨ was 0.8, 1, 2 and 4 IU, the detection results of FⅨ titer were all lower than those of samples without heparin. When the ATⅢ content was 0, 0.1, 0.5 and 1 IU, the FⅨ titer of the samples was consistent. Conclution When the content of heparin or heparin/ATⅢ in the product is less than or equal to 0.5 IU per IU of FⅨ, the step of protamine sulfate adding could be omitted as it has little effect on FⅨ activity. When >0.5 IU per IU of FⅨ, however, protamine sulfate adding, to neutralize heparin, is necessary before FⅨ activity testing.
Authors and Affiliations
Changyong JIAN, Miaomiao DOU, chen CHEN, An ZHOU, Chao GAO, Cuiping ZHANG
Keywords
Related Articles
- EP ID EP738275
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.06.017
- Views 5
- Downloads 0
Concordance of identifying the presence or absence of KIR genes by Flow-rSSO and PCR-SBT methods: a comparative study
Objective To study the concordance of identifying the presence or absence of KIR genes using flow reverse sequence-specific oligonucleotide probe (Flow-rSSO) hybridization and sequencing based typing-PCR (PCR-SBT) method...
Identification and pedigree investigation of B(A) and cisAB blood groups
Objective To investigate the serological and molecular genetic characteristics of B(A) and cisAB blood groups discovered in our laboratory. Methods ABO blood group serology and genetic tests were used to identify blood g...
A survey on professional identity and job satisfaction of blood transfusion physicians and its influencing factors, in Tianjin
Objective To investigate the status of professional identity and job satisfaction of blood transfusion physicians in clinical in Tianjin, and to analyze the influencing factors. Methods The status of professional identit...
The Progress of Red Blood Cells Based Drug Delivery Systems
Red blood cells(RBCs), as the most abundant types of blood cells in the blood, have several advantages as a drug delivery system due to their ultra-long circulation half-life, good biocompatibility, and biodegradability....
Evaluation of hierarchical teaching model in advanced training of therapeutic blood component apheresis
Objective To explore the efficiency of hierarchical teaching model in advanced training of therapeutic blood component apheresis. Methods A total of 76 students who participated in the advanced training(≥3 months) of the...