Influence of Environmental Factors on Activities of Bacterial Population Associated With Rhizospheric Soil of Wheat Crop
Journal Title: International Journal of Environment and Climate Change - Year 2017, Vol 7, Issue 3
Abstract
Aim: To study the effect of environmental factors on the activities of soil microbial population in the rhizospheric soil of wheat crop. Study Design: An agroclimatic study was carried out to study the effect of environmental alterations on the activity of soil bacteria in a multifactor climate change experiment in which wheat crop was grown under field conditions and under temperature gradient tunnel maintained at different and higher temperature. Attempt was made to screen high CO2 and high temperature tolerant diazotrophic bacteria from wheat rhizosphere. Place of Study: Department of Microbiology, PAU, Ludhiana. Methodology: Nitrogen fixing bacteria were isolated on Jensen’s medium using serial dilution spread plate technique. The bacterial isolates were characterized biochemically using standard techniques as described in Bergey’s Manual of Determinative Bacteriology.The isolates were also assessed for their ability to produce indole acetic acid, siderophores production, ammonia excretion, qualitative phosphate solubilization and solubilization of phosphate by quantitative method. Results: A total of 21 different nitrogen fixing bacteria were isolated from rhizospheric soil samples of wheat crop grown under field conditions and under temperature gradient tunnel. Out of 21 isolates, 8 isolates were able to grow upto 20% concentration of CO2 and 7 isolates showed growth upto 60°C. Tolerance to high CO2 and high temperature was observed to more in the bacteria isolated from the rhizospheric soil of wheat crop grown under temperature gradient tunnel. Functional characterization of these isolates showed that the isolate WT5 had significantly higher IAA production (44.3 µg/ml) after 5 days of incubation in the medium supplemented with tryptophan. Among these 21 isolates, six were found to have P-solubilizing diazotrophic trait and five were found to show siderophore production on CAS agar plates. The amount of ammonia excretion was non-significant among the isolates and was in the range of 1.14-3.70 µg/ml. The isolate WF6 was found to be the best isolate in terms of the functional characteristics and tolerance to high CO2 and temperature levels. Conclusion: Results indicate that alterations in environmental factors may cause changes in activities of bacterial populations. These results illustrate the potential for complex community changes in terrestrial ecosystems under climate change scenarios that alter multiple factors simultaneously.
Authors and Affiliations
Jupinder Kaur, S. K. Gosal
Keywords
Related Articles
- EP ID EP350463
- DOI 10.9734/BJECC/2017/35188
- Views 110
- Downloads 0
A Study of Urban Heat Island using “Local Climate Zones” – The Case of Singapore
Aims: The study of urban heat islands and traditionally relies on simplistic descriptors such as “urban” and “rural”. While these descriptors may be evocative of the landscape, they are insufficient in providing informat...
Phytodiversity of Herbaceous Vegetation in Disturbed and Undisturbed Forest Ecosystems of Pahalgam Valley, Kashmir Himalaya, India
The study investigated the comparative assessment of seasonal herbaceous diversity at disturbed and undisturbed forest sites at Pahalgam and Betab valley of Kashmir. The results revealed that Shannon’s diversity attained...
Crop Co-efficient Values of Sunflower for Different Growth Stages by Lysimeter Study
The experiment was conducted on sunflower (variety BARI Surjomukhi-2) crop during the month of mid-November, 2014 to mid-March, 2015, in a lysimeter (dimension:1 m X 1 m X 1 m size) to measure crop evapotranspiration (ET...
Hydrobiological Assessment of the High Mountain Kolsay Lakes (Kungey Alatau, Southeastern Kazakhstan) Ecosystems in Climatic Gradient
Aims: The aim of this study is to assess the ecological status of the Kolsay high mountain lakes according to their hydrochemical parameters and structure of phytoplankton communities and reveal the altitude climatic con...
Greenhouse Gas Emission Determinants in Nigeria: Implications for Trade, Climate Change Mitigation and Adaptation Policies
This study investigated and analyzed the determinants of Carbon Dioxide (CO2) emission in Nigeria. The study relied on secondary data from World Bank and Central Bank of Nigeria covering 40 years (1970-2009). The data we...