INFLUENCE OF EXPLANTS TYPE AND PLANT GROWTH REGULATORS ON IN VITRO MULTIPLE SHOOTS REGENERATION OF VANILLA PLANIFOLIA
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 2
Abstract
The frequency of shoot bud regeneration was influenced by the type of explant, genotype and concentrations of plant growth regulators. An efficient in vitro multiplication protocol was developed through excised shoot tip and nodal segment of Vanilla planifolia and was studied with an aim to select best explant and effect of different plant growth regulators (PGRs) alone or in combination on multiple shoots production from different explants of V. planifolia. Explants viz. shoot tips and nodal segments of vanilla were cultured on MS medium augmented with different concentrations of BAP/Kinetin/NAA. Among the various concentrations tested, MS medium supplemented with 3.0 mg/L BAP and 1.0 mg/L NAA was found to be the best for maximum shoot bud differentiation. Maximum number of multiple shoots per explant (11.60 shoots/explant) was obtained from nodal segments.
Authors and Affiliations
RUBY SHARMA, SUNIL BORA
Keywords
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