INFLUENCE OF EXPLANTS TYPE AND PLANT GROWTH REGULATORS ON IN VITRO MULTIPLE SHOOTS REGENERATION OF VANILLA PLANIFOLIA
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 2
Abstract
The frequency of shoot bud regeneration was influenced by the type of explant, genotype and concentrations of plant growth regulators. An efficient in vitro multiplication protocol was developed through excised shoot tip and nodal segment of Vanilla planifolia and was studied with an aim to select best explant and effect of different plant growth regulators (PGRs) alone or in combination on multiple shoots production from different explants of V. planifolia. Explants viz. shoot tips and nodal segments of vanilla were cultured on MS medium augmented with different concentrations of BAP/Kinetin/NAA. Among the various concentrations tested, MS medium supplemented with 3.0 mg/L BAP and 1.0 mg/L NAA was found to be the best for maximum shoot bud differentiation. Maximum number of multiple shoots per explant (11.60 shoots/explant) was obtained from nodal segments.
Authors and Affiliations
RUBY SHARMA, SUNIL BORA
Heterosis for Grain Yield and its Important Components in Aromatic Rice (Oryza Sativa L.)
Eight genotypes consisting three Basmati varieties viz, Basmati Kasturi, Basmati 370 and Basmati with five Manipur scented rice landraces viz, Chakhao Poireiton, Chakhao, Chakhao Amubi, Ching Chakhao and Chakhao Angangba...
New Opportunity to Farmers: the Commodity Hedge
In an ironic manner, we get news by the NCRB (National Crime Records Bureau), as 16,632 farmers, including 2,369 women, killed themselves last year (2014), constituting 14.4% of the total number of suicides in the countr...
Farmer's Perception of Climate Change in the Kosi - Watershed of Central Himalayan Region
Himalayan ecosystem supports varieties of livelihood options, such as terraced farming, agro forestry and orchards. The main source of rural livelihood and food security in the Indian Himalayan region is, rain-fed agricu...
Impact on Socio-economic Status after Induction of Improved Goat Farming Practices among Tribals of Abu Road, Rajasthan
The aim was to study the socio-economic assessment of improved goat farming. The study, conducted as per the MOU with MPOWER, adopted 6 villages, namely, Siyawa, Meen, Taleti, Jambudi, Deri and Surpagla of block Abu Road...
Performance of Turmeric (Curcuma Longa L) Varities in Gangetic Alluvial Plains of West Bengal
A field experiment was conducted in the year 2014-15 and 2015-16, at Horticulture research farm, Jaguli, Bidhan Chandra Krishi Viswavidyalaya, Nadia, West Bengal, in factorial randomized block design with four replicatio...