INFLUENCE OF POLYMERIC AND RUBBER MATERIALS ON HYDROCARBON-OXIDIZING BACTERIA
Journal Title: Мікробіологія і біотехнологія - Year 2019, Vol 0, Issue 2
Abstract
Polymeric materials are widely used in buildings and various industrial branches and consequently their persistence to microbial remediation draws scientist’s attention. Aim. To determin the influence of foamed polyethylene, ethylenevinylacetate and rubber on hydrogen-oxidizing bacteria: Pseudomonas pseudoalcaligenes 109, Rhodococcus erythropolis 102, Bacillus subtilis 138, isolated from damaged protective gas pipeline coatings. Methods. Bacterial quantity was determined by the serial dilution method; destruction index – gravimetrically for the weight loss; enzymatic activity – by the spectrophotometry on the KFK-3; changes in the chemical compound – by the IR Furrier spectroscopy on the TENSOR-37. Results. It was shown that in the presence of the studied materials as a sole carbon source catalase activity of P. pseudoalcaligenes 109, R. erythropolis 102 were decreased in 1.5–3.2 and 2.8–3.9, respectively, and in B. subtilis 138 were increased in 1.4–2.5 times than in control (Tauson media inoculated with bacteria and without material). Lipase activity of B. subtilis 138 and R. erythropolis 102 under the presence of studied materials were decreased in 1.2–3.8 times than in control. The study (by the IR-spectroscopy) of the polymeric and rubber materials components after the bacterial influence have shown that content of materials was not chemically changed. During 90 days of exposition the destruction coefficient of rubber was 0.4–0.6% (weight loss 10±0.9 and 16.6±2.6 mg depend on bacterial culture). For foamed polyethylene and ethylenevinylacetate destruction coefficient was not significant (weight loss up to 0.9±0.01 mg). Conclusions. Studied materials had been put in Tauson media as a sole carbon and energy source promoted the decrease of catalase activity in R. erythropolis 102 and P. pseudoalcaligenes 109, as well as lipolytic activity decreasing in R. erythropolis 102 and B. subtilis 138.
Authors and Affiliations
D. R. Abdulina, Zh. P. Kopteva, A. E. Kopteva, M. Ya. Vortman
Keywords
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- EP ID EP670083
- DOI 10.18524/2307-4663.2019.2(46).169092
- Views 103
- Downloads 0
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