Influences of growth regulators on callus induction, ephedrine and pseudoephedrine contents and chemical analysis of mature embryo of Ephedra strobilacea Bunge
Journal Title: Advances in Agriculture & Botanics - International Journal of the Bioflux Society - Year 2011, Vol 3, Issue 1
Abstract
An experiment was carried out to develop a protocol for callus induction of Ephedra strobilacea Bunge from embryo. Different types and concentrations of plant growth regulators were tested in order to obtain the best callus formation, and the following hormonal ranges: 2, 4-D: 1 Kin: 1 (mg L[sup]-1[/sup]) and 2, 4-D: 1 BA: 0.1 (mg L[sup]-1[/sup]) had showed the highest callus induction and the highest fresh weight, respectively. Meanwhile, ephedrine and pseudo-ephedrine contents in callus were analyzed and we found that at NAA: 1 Kin: 1 (mg L[sup]-1[/sup]) range the ephedrine and pseudo-ephedrine contents had significantly increased compared to the other treatments.
Authors and Affiliations
Badrossadat Mousavi, Ali Parsaeimehr, Neda Irvani
Keywords
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