Influencing factors of the repeat reactive (RR) rates of minipools implicated in minipool(MP) nucleic acid testing(NAT) in Xiamen
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 7
Abstract
Objective To analyze the influencing factors of the repeat reactive (RR) rates of minipools implicated in minipool (MP) nucleic acid testing(NAT) in Xiamen Blood Center, in order to provide reference for NAT. Methods Samples of blood donors from January 1, 2019 to October 31, 2022 were collected in Xiamen Blood Center and tested by MP-NAT(pools of six). Statistical analysis and comparison of MP-NAT RR rates was performed among different years, testers, reagent batches, instrument combinations, CT values of MP-NAT reactive pools and sample backgrounds. Results A total of 234 715 blood samples were tested by MP-NAT, and 428 pools were reactive, in which 248 pools were individual-donor NAT reactive, with a MP-NAT RR rate of 57.9%. The difference of MP-NAT RR rates were not statistically significant among different years, testers, reagent batches, instrument combinations, and sample backgrounds (P> 0.05). The difference of MP-NAT RR rates among different CT values of MP-NAT reactive pools was statistically significant (χ2=69.587, P<0.05). Significantly abnormal RR rate accurred in two months in 2022, and returned to normal after timely handling. Conclusion The MP-NAT RR rates is one of the important indicators to monitor the quality of NAT. Once there is a significant change in the MP-NAT RR rates, comprehensive analysis and timely handling should be carried out to ensure the quality of blood detection.
Authors and Affiliations
Jiahuang WU, Shucheng ZHUANG, Bin PEI, Shanhai OU
Effect of recombinant human coagulation factor Ⅶa combined with Bakri balloon compression on oxidative stress and coagulation in patients with refractory postpartum hemorrhage
Objective To investigate the effects of recombinant human coagulation factor Ⅶa combined with Bakri balloon compression on oxidative stress and coagulation in patients with refractory postpartum hemorrhage. Methods Prosp...
Application of improved comparability verification method in performance verification of instruments and equipment
Objective To explore the viability of improved comparability verification method for the performance verification of automatic biochemical analyzer in blood centers. Methods According to the relevant requirements of User...
Comparative study of the walk-in and appointment mode of apheresis platelets donation in Guangzhou
Objective To discuss the influence of apheresis platelets donation mode transformation, from walk-in to appointment, on apheresis platelets donation, donor retention and donation service quality. Methods The comparative...
Countermeasures of adverse reactions to blood donation in low-weight blood donors for the MCS+ blood cell separator UPP procedure
Objective To reduce the occurrence of adverse reactions to blood donation during platelet collection in female blood donors with low body weight and high platelet count, so as to improve the comfortableness during platel...
Application of MALDI-TOF MS in clinical difficult blood group typing
Objective To explore the application of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) in the genotyping of difficult blood typing samples, and to provide evidence for clinica...