Inheritance of glyphosate and glufosinate resistance in T1-T2 generations of biotechnological canola (Brassica napus L.) plants
Journal Title: Вісник Українського товариства генетиків і селекціонерів - Year 2015, Vol 13, Issue 1
Abstract
Aim. To obtain the biotechnological canola plants using the introduction of epsps and bar genes in the same cassette and investigate both inheritance of target genes and resistance to corresponding herbicides in Т1–Т2 generations. Меthods. Agrobacterium tumefaciens-mediated genetic transformation,molecular biological (PCR, RT-PCR),genetic (study of segregation for phosphinothricin resistance under aseptic conditions), physiological (evaluation of herbicide resistance, determination of biomass and total soluble proteins (TSP)). Results. The transgenic plants with epsps and bar genes of resistance to herbicides based on glyphosate and glufosinate have been obtained. The integration of target genes into nuclear genome and their activities on transcriptional level were shown. Resistance of biotechnological plants to herbicide treatments was proved in greenhouse. Stable and linked inheritance of transgenes was demonstrated. It was shown that transgenic plants grown in greenhouse and not treated with herbicide do not differ from the initial untransformed ones in biomass and TSP content in leaves, when they were. It was found that spraying of biotechnological plants with herbicides does not affect the growth rate, biomass, and TSP content. Conclusions. The biotechnological plants with genes of herbicide resistance to glyphosate (epsps gene) and glufosinate (bar gene) were obtained. Transgene expression was confirmed for Т1–Т2 generations. It did not influence biomass production and TSP content in transgenic plants under greenhouse conditions.Кeywords: Brassica napus canola, epsps, bar, glyphosate, glufosinate.
Authors and Affiliations
L. O. Sakhno, I. K. Komarnitsskii, M. V. Кuchuk
Keywords
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